Isaenko A A, Nemtsov Iu V, Tsareva A A
Tsitologiia. 1990;32(7):736-40.
The karyological analysis of the cell line 4647 used for production of a killed vaccine to Hepatitis A virus was run in the 98th, 107th, 117th and 127th passages by the routine and C, G, and Ag methods of differential chromosome staining. A considerable balancing of the chromosome composition at 107-127 passage levels is shown. The cells of line 4647 present a significant heterogeneity, as to the number of chromosomes, and do not belong to any distinct modal class. The modal number of chromosomes ranged from 61 to 66 and from 121 to 125 for hyperdiploid and polyploid cells, respectively. The stable modal class of cells was established in the tetrasomic region, when culturing in the medium containing 10% CS from the 107th passage, and in the medium containing 10% FBS from the 117th passage, which conforms to one of the WHO requirements asserted to the substrate cells.
对用于甲型肝炎病毒灭活疫苗生产的4647细胞系进行了核型分析，采用常规及C、G和Ag差异染色体染色方法，分别在第98、107、117和127代进行分析。结果显示，在第107 - 127代水平，染色体组成有相当程度的平衡。4647细胞系的细胞在染色体数目上表现出显著的异质性，不属于任何明确的众数类别。超二倍体细胞和多倍体细胞的染色体众数分别为61至66条和121至125条。当从第107代开始在含10%小牛血清的培养基中培养，以及从第117代开始在含10%胎牛血清的培养基中培养时，在四体区域建立了稳定的细胞众数类别，这符合世界卫生组织对底物细胞规定的要求之一。
