Seifertová M
Neoplasma. 1984;31(1):3-13.
The karyotype of L1210 mouse leukemia cells was studied using G-banding, C-banding and Ag-staining methods. In modal chromosome number and G-banding patterns we were interested in the differences between line A and B carried by DBA/2 mice in our laboratory. Both lines had the modal chromosome number of 38. Lines differed significantly in the average number of nine chromosomes per karyotype, whereas in marker chromosomes significant difference was found only in M2 and M6. Marker M1 t(5, 12) was present in 95% of cells in line A and 100% of cells in line B. More than 50% of cells in both lines contained M2, M11, M13 and M14. X-Chromosome and chromosome No. 4 were most frequently absent. More than 80% of cells in both lines had only one chromosome No. 5, and trisomy of chromosome No. 15 was found in 50% of cells in line A and 40% in line B. Values for disomy were low for all chromosomes in both lines. Marker M1 t(5, 12) possessed C-banding material at the proximal end of translocated chromosome No. 5 and NOR at the proximal end of translocated chromosome No. 12. Marker M2 t(1, 12) without NOR had the C-banding material located at the proximal end of chromosome No. 1.
运用G显带、C显带和银染法研究了L1210小鼠白血病细胞的核型。我们关注的是本实验室DBA/2小鼠所携带的A系和B系在染色体众数和G显带模式上的差异。两系的染色体众数均为38。两系在每个核型中九条染色体的平均数上存在显著差异，而在标记染色体方面，仅在M2和M6上发现显著差异。标记M1 t(5, 12)在A系95%的细胞和B系100%的细胞中存在。两系中超过50%的细胞含有M2、M11、M13和M14。X染色体和4号染色体最常缺失。两系中超过80%的细胞只有一条5号染色体，A系50%的细胞和B系40%的细胞中发现15号染色体三体。两系所有染色体的二体数值都很低。标记M1 t(5, 12)在易位的5号染色体近端具有C带物质，在易位的12号染色体近端具有核仁组织区。标记M2 t(1, 12)没有核仁组织区，其C带物质位于1号染色体近端。
