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Adv Exp Med Biol. 2011;700:76-84. doi: 10.1007/978-1-4419-7823-3_8.
From analysis of deep-sequencing data it is apparent that sequence differences occur between the genome and miRNAs. Changes from genomic A to an apparent G in miRNA can be accounted for by the editing activity of ADARs. Questions that arise from this observation are: How many miRNAs are edited and to what frequency? Is there a specific step in the biogenesis of miRNAs that is preferentially susceptible to editing by ADARs? However the key question is whether editing affects the downstream activity of miRNAs. Despite much evidence that miRNAs are edited, critical examination of the functional data shows a dearth of examples where editing has been demonstrated to actually affect the downstream miRNA activity in vivo. Even where it is demonstrated that RNA editing can affect biogenesis or targeting of a particular miRNA, effects may be limited by redundancy within the miRNA network.
从深度测序数据分析中明显看出，基因组和 miRNA 之间存在序列差异。miRNA 中从基因组 A 到表观 G 的变化可以通过 ADARs 的编辑活性来解释。由此观察产生的问题是：有多少 miRNA 被编辑，频率是多少？miRNA 生物发生过程中是否存在特定步骤更容易受到 ADAR 的编辑？然而，关键问题是编辑是否会影响 miRNA 的下游活性。尽管有大量证据表明 miRNA 被编辑，但对功能数据的严格检查表明，实际上证明编辑会影响体内 miRNA 下游活性的例子很少。即使表明 RNA 编辑可以影响特定 miRNA 的生物发生或靶向，这种影响也可能受到 miRNA 网络中的冗余限制。
