Steinhelper M E, Lanson N A, Dresdner K P, Delcarpio J B, Wit A L, Claycomb W C, Field L J
Cold Spring Harbor Laboratory, New York 11724.
Am J Physiol. 1990 Dec;259(6 Pt 2):H1826-34. doi: 10.1152/ajpheart.1990.259.6.H1826.
Transgenic mice expressing atrial natriuretic factor-SV40 T-antigen fusion genes (ANF-TAG) developed unilateral right atrial tumors composed of differentiated dividing cardiomyocytes. The atrial tumors could be propagated as transplantable tumor lineages in syngeneic animals. Cardiomyocytes derived from ANF-TAG atrial tumors did not proliferate in tissue culture. However, cardiomyocytes derived from the transplantable tumor lines proliferated in culture, and these proliferating cardiomyocytes could be passaged in culture and recovered from frozen stocks. Cardiomyocytes from either tumor source were highly differentiated as determined by diverse functional and structural criteria. The cells continued to express numerous cardiac-specific proteins and retained ultrastructural features characteristic of cardiomyocytes including well-formed myofibrils, transverse tubules, and intercalated disks. In addition, the cultured cells displayed spontaneous electrical and contractile activities. These atrial tumor cardiomyocytes are a novel experimental resource for the identification of genes regulating the cardiomyocyte cell cycle.
表达心钠素 - SV40 T抗原融合基因（ANF - TAG）的转基因小鼠产生了由分化的分裂心肌细胞组成的单侧右心房肿瘤。心房肿瘤可作为可移植肿瘤谱系在同基因动物中传播。源自ANF - TAG心房肿瘤的心肌细胞在组织培养中不增殖。然而，源自可移植肿瘤系的心肌细胞在培养中增殖，并且这些增殖的心肌细胞可以在培养中传代并从冷冻保存中复苏。根据多种功能和结构标准确定，来自任何一种肿瘤来源的心肌细胞都高度分化。这些细胞继续表达大量心脏特异性蛋白，并保留了心肌细胞特有的超微结构特征，包括结构良好的肌原纤维、横小管和闰盘。此外，培养的细胞表现出自发性电活动和收缩活动。这些心房肿瘤心肌细胞是鉴定调节心肌细胞细胞周期基因的新型实验资源。
