Afridi Hassan Imran, Kazi Tasneem Gul, Kazi Naveed, Kandhro Ghulam Abbas, Baig Jameel Ahmed, Shah Abdul Qadir, Khan Sumaira, Kolachi Nida Fatima, Wadhwa Sham Kumar, Shah Faheem
Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan.
Clin Lab. 2011;57(5-6):387-96.
The aim of the present study was to compare the level of calcium (Ca), magnesium (Mg), potassium (K), and sodium (Na) in biological samples (serum, blood, urine, and scalp hair) of patients suffering from different types of viral hepatitis (A, B, C, D, and E; n = 521) of both genders, ages ranged between 31-45 years. For comparative study, 255 age-matched subjects of both genders, residing in the same city, were selected as controls.
The elements in the biological samples were analyzed by flame atomic absorption spectrophotometry prior to microwave-assisted acid digestion. The validity and accuracy of the methodology was checked by using certified reference materials (CRMs) and values obtained by conventional wet acid digestion method on the same CRMs.
The results of this study showed that the mean value of Na was higher in blood, sera, and scalp hair samples of hepatitis patients, while Ca, Mg and K levels were found to be lower than age-matched control subjects. The urinary levels of these elements were found to be higher in the hepatitis patients than in the age-matched healthy controls (p < 0.05).
These results are consistent with data reported in literature, confirming that the deficiency of essential mineral elements and sodium overload can directly cause lipid peroxidation and eventually hepatic damage.
本研究旨在比较年龄在31至45岁之间的不同类型病毒性肝炎（甲型、乙型、丙型、丁型和戊型；n = 521）患者的生物样本（血清、血液、尿液和头皮毛发）中钙（Ca）、镁（Mg）、钾（K）和钠（Na）的水平。为进行对比研究，选取了居住在同一城市的255名年龄匹配的男女受试者作为对照。
在微波辅助酸消解之前，通过火焰原子吸收分光光度法分析生物样本中的元素。通过使用有证标准物质（CRMs）以及通过传统湿酸消解方法在相同CRMs上获得的值来检验该方法的有效性和准确性。
本研究结果表明，肝炎患者血液、血清和头皮毛发样本中的钠平均值较高，而钙、镁和钾水平低于年龄匹配的对照受试者。发现这些元素的尿液水平在肝炎患者中高于年龄匹配的健康对照（p < 0.05）。
这些结果与文献报道的数据一致，证实必需矿物质元素缺乏和钠过载可直接导致脂质过氧化并最终造成肝损伤。
