Afridi Hassan Imran, Kazi Tasneem Gul, Kazi Naveed, Shah Abdul Qadir, Khan Sumaira, Kolachi Nida Fatima, Wadhwa Sham Kumar, Shah Faheem
Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
Clin Lab. 2012;58(1-2):7-18.
Rheumatoid Arthritis is a chronic inflammatory disease resulting in joint inflammation (particularly joints of hands, wrists, feet, knees, ankles, and shoulder) that is manifested by swelling and functional impairment.
This study was designed to compare the levels of calcium (Ca), magnesium (mg), potassium (K), and sodium (Na) in four biological samples (scalp hair, serum, blood, and urine) of patients with rheumatoid arthritis (RA) as compared to referent subjects of both genders who do have not arthritis problems. All patients and referents were divided in two age groups, (46-60) and (61-75) years. A microwave assisted wet acid digestion procedure was used for acid digestion of biological samples. The digests of all biological samples were analysed for Ca, Mg, K, and Na by flame atomic absorption spectrometry (FAAS). The proposed method was validated by using conventional wet digestion of the same sub samples and certified reference samples of hair, serum, blood, and urine.
The results indicated significantly lower levels of Ca, Mg, and K in the biological samples (blood, serum, and scalp hair) of male and female rheumatoid arthritis patients when compared to referents of both genders, whereas the levels of Na were found to be high in blood and urine samples of patients as compared to nonrheumatic referents.
These data represent a guide for clinicians and other professionals who will be investigating the deficiency of essential micronutrients in biological samples (scalp hair, serum, and blood) of rheumatoid arthritis patients.
类风湿性关节炎是一种慢性炎症性疾病，会导致关节炎症（尤其是手部、腕部、足部、膝盖、脚踝和肩部的关节），表现为肿胀和功能障碍。
本研究旨在比较类风湿性关节炎（RA）患者与无关节炎问题的男女对照受试者的四种生物样本（头皮毛发、血清、血液和尿液）中的钙（Ca）、镁（Mg）、钾（K）和钠（Na）水平。所有患者和对照者被分为两个年龄组，（46 - 60）岁和（61 - 75）岁。采用微波辅助湿酸消解程序对生物样本进行酸消解。通过火焰原子吸收光谱法（FAAS）分析所有生物样本消解液中的Ca、Mg、K和Na。通过对相同子样本以及头发、血清、血液和尿液的标准参考样本进行常规湿消解来验证所提出的方法。
结果表明，与男女对照者相比，类风湿性关节炎患者的生物样本（血液、血清和头皮毛发）中的Ca、Mg和K水平显著较低，而与非风湿性对照者相比，患者血液和尿液样本中的Na水平较高。
这些数据为临床医生和其他专业人员提供了指导，他们将研究类风湿性关节炎患者生物样本（头皮毛发、血清和血液）中必需微量营养素的缺乏情况。
