采用B.R.A.H.M.S Kryptor上的既定方法对西门子ADVIA Centaur的新型降钙素原检测法进行评估。

Evaluation of a new procalcitonin assay for the Siemens ADVIA Centaur with the established method on the B.R.A.H.M.S Kryptor.

作者信息

Sanders Robert-Jan, Schoorl Marianne, Dekker Ellen, Snijders Dominic, Boersma Wim G, Ten Boekel Edwin

机构信息

Department of Clinical Chemistry, Hematology and Immunology, Medical Center Alkmaar, The Netherlands.

出版信息

Clin Lab. 2011;57(5-6):415-20.

PMID:21755834

Abstract

BACKGROUND

In this study, we compared the B.R.A.H.M.S Kryptor procalcitonin (PCT) assay with the newly developed ADVIA Centaur B.R.A.H.M-S PCT assay. Furthermore, the long-term stability of PCT at - 20 degrees C was assessed.

METHODS

Samples from 97 patients with lower respiratory tract infections were retested on both systems and compared with Passing-Bablok regression over two clinically relevant cutoff ranges for PCT, 0 - 2.0 microg/L and > 2.0 microg/L.

RESULTS

After storage for 2.5 to 4 years, PCT levels in patient sera declined only 3.7%. Passing-Bablok regression analysis of the total sample range (n = 97) showed that both methods correlated well (r = 0.9944), although with a deviation from the line of identity (y = 0.880x - 0.025 microg/L). Comparison of both methods within the clinically important interval of 0 - 2.0 microg/L showed acceptable correlation (y = 0.943x + 0.010 microg/L).

CONCLUSIONS

The ADVIA Centaur B.R.A.H.M.S PCT assay showed good correlation with the established Kryptor method. Therefore, this new technique can be used in clinical routine with the same clinical interpretation.

摘要

背景

在本研究中，我们将B.R.A.H.M.S Kryptor降钙素原（PCT）检测法与新开发的ADVIA Centaur B.R.A.H.M.S PCT检测法进行了比较。此外，还评估了PCT在-20℃下的长期稳定性。

方法

对97例下呼吸道感染患者的样本在两个系统上重新进行检测，并在PCT的两个临床相关临界值范围（0 - 2.0μg/L和>2.0μg/L）内与Passing-Bablok回归法进行比较。

结果

在储存2.5至4年后，患者血清中的PCT水平仅下降了3.7%。对整个样本范围（n = 97）进行的Passing-Bablok回归分析表明，两种方法相关性良好（r = 0.9944），尽管与恒等线存在偏差（y = 0.880x - 0.025μg/L）。在0 - 2.0μg/L这一临床重要区间内对两种方法进行比较，显示出可接受的相关性（y = 0.943x + 0.010μg/L）。