运用巢式甲基化特异性聚合酶链反应检测技术对卵巢癌中阿片样物质结合蛋白/细胞黏附分子样基因甲基化状态的研究
A study of the methylation status of opioid binding protein/cell adhesion molecule-like gene in ovarian cancer using nested methylation-specific polymerase chain reaction detection.
作者信息
Zhou Feng, Cao Xingjian, Liu Manhua, Wang Ying, Tao Guohua
机构信息
Department of Clinical Chemistry, Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China.
出版信息
Clin Lab. 2011;57(5-6):421-4.
BACKGROUND
The goal was to improve the methylation-specific PCR (MSP) method and investigate the methylation status of the OPCML gene in carcinoma tissues from ovarian cancer patients.
METHODS
MSP and nested MSP methods were used to examine the methylation status of the OPCML gene promoter in ovarian cancer, borderline tumor, and normal ovary tissues.
RESULTS
Methylation of the OPCML gene was detected in 58.3% (14/24) and 83.3% (20/24) of the specimens from ovarian cancer patients using MSP and nested MSP methods, respectively. No methylation was observed in normal ovarian tissues using either method.
CONCLUSIONS
The modified nested MSP method showed better sensitivity. The methylation of the OPCML gene was significantly higher in ovarian cancer than in normal tissue. The detection of OPCML gene methylation could serve as one of the molecular markers for the diagnosis of ovarian cancer.
背景
目的是改进甲基化特异性PCR（MSP）方法，并研究卵巢癌患者癌组织中OPCML基因的甲基化状态。
方法
采用MSP和巢式MSP方法检测卵巢癌、交界性肿瘤和正常卵巢组织中OPCML基因启动子的甲基化状态。
结果
分别采用MSP和巢式MSP方法在58.3%（14/24）和83.3%（20/24）的卵巢癌患者标本中检测到OPCML基因甲基化。两种方法在正常卵巢组织中均未观察到甲基化。
结论
改良的巢式MSP方法显示出更好的敏感性。OPCML基因在卵巢癌中的甲基化显著高于正常组织。检测OPCML基因甲基化可作为卵巢癌诊断的分子标志物之一。
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