Rustandi R R, Snyder S W, Feezel L L, Michalski T J, Norris J R, Thurnauer M C, Biggins J
Chemistry Division, Argonne National Laboratory, Illinois 60439.
Biochemistry. 1990 Sep 4;29(35):8030-2. doi: 10.1021/bi00487a006.
The electron spin polarized (ESP) electron paramagnetic resonance (EPR) signal observed in spinach photosystem I (PSI) particles was examined in preparations depleted of vitamin K1 by solvent extraction and following biological reconstitution by the quinone. The ESP EPR signal was not detected in the solvent-extracted PSI sample but was restored upon reconstitution with either protonated or deuterated vitamin K1 under conditions that also restored electron transfer to the terminal PSI acceptors. Reconstitution using deuterated vitamin K1 resulted in a line narrowing of the ESP EPR signal, supporting the conclusion that the ESP EPR signals in the reconstituted samples arise from a radical pair consisting of the oxidized PSI primary donor, P700+, and reduced vitamin K1.
在通过溶剂萃取去除维生素K1并随后用醌进行生物重建的菠菜光系统I（PSI）颗粒制剂中，对观察到的电子自旋极化（ESP）电子顺磁共振（EPR）信号进行了检测。在溶剂萃取的PSI样品中未检测到ESP EPR信号，但在质子化或氘代维生素K1重建后恢复，且这些条件也恢复了向PSI末端受体的电子转移。使用氘代维生素K1重建导致ESP EPR信号的线宽变窄，支持了重建样品中的ESP EPR信号源自由氧化的PSI初级供体P700 +和还原的维生素K1组成的自由基对的结论。
