Zhao Jing-yu, Chai Jia-ke, Song Hui-feng, Xu Ming-huo
Department of Burns & Plastic Surgery, First Affiliated Hospital, General Hospital of PLA, Beijing 100048, China.
Zhonghua Yi Xue Za Zhi. 2012 Jun 26;92(24):1719-22.
To explore the effects of hyaluronic acid (HA) on biomechanical properties for porcine acellular dermal matrix (PADM) plus thin skin autograft after transplantation.
The dorsa of 10 Japanese white rabbits were symmetrically divided into four areas of A-D by random grouping. Full-thickness skin defects were created in Groups A-C while Group D was blank with normal skin. Operations were performed in Group A: implant with HA + PADM + thin skin autografts, Group B: implant with PADM + thin skin autografts and Group C: skin autografts group. Histological examination of specimen was performed at Day 56 postoperatively. And the biomechanical properties such as relaxation and stress-strain properties of grafts were recorded.
The structure of PADM was found to be basically intact by hematoxylin and eosin E dyeing in Groups A and B. In Group A, dense fiber structure could be observed. Lots of regularly arranged collagenous fibers and new blood capillaries were grown into the dermal matrix with sparsely distributed inflammatory cells. In Group B, acellular dermal matrix became clustered with a small amount of invaded fibroblasts. And there was a high expression of inflammatory cells. The biomechanic performances of transplanted skin were: Group A's curve was mostly close to that of Group D's, Group B's curve was the most further from that of Group D's (P = 0.001) and Group C's curve stayed between Groups A and B. Under the same strain, the stress of Groups A-D was (87 ± 8), (115 ± 9), (63 ± 7) and (81 ± 4) kPa respectively. No significant difference of stress existed between these two groups (P = 0.838). There was significant difference of stress between Groups B/C and D (P = 0.001 and P = 0.009).
Topical hyaluronic acid may be used to enhance the biomechanics performances of transplanted skin.
探讨透明质酸（HA）对猪脱细胞真皮基质（PADM）复合自体薄皮移植后生物力学性能的影响。
将10只日本大耳白兔背部对称随机分为A - D 4个区域。A - C组制造全层皮肤缺损，D组为正常皮肤作为空白对照。A组手术方式为植入HA + PADM + 自体薄皮；B组为植入PADM + 自体薄皮；C组为单纯自体薄皮移植组。术后第56天取标本进行组织学检查，并记录移植皮片的松弛、应力 - 应变等生物力学性能。
A、B组苏木精 - 伊红染色显示PADM结构基本完整。A组可见致密的纤维结构，大量规则排列的胶原纤维和新生毛细血管长入真皮基质，炎症细胞散在分布。B组无细胞真皮基质聚集，有少量成纤维细胞侵入，炎症细胞表达较高。移植皮肤的生物力学性能表现为：A组曲线最接近D组，B组曲线与D组相差最远（P = 0.001），C组曲线介于A、B组之间。在相同应变下，A - D组应力分别为（87 ± 8）、（115 ± 9）、（63 ± 7）和（81 ± 4）kPa，A与C组应力差异无统计学意义（P = 0.838），B、C组与D组应力差异有统计学意义（P = 0.001和P = 0.009）。
局部应用透明质酸可提高移植皮肤的生物力学性能。
