Zhao Jing-yu, Chai Jia-ke, Song Hui-feng, Xu Ming-huo
Department of Burn and Plastic Surgery, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China.
Zhonghua Yi Xue Za Zhi. 2012 May 29;92(20):1428-30.
To explore the effect on biomechanical compliance after an addition of hyaluronic acid (HA) in transplantation of porcine acellular dermal matrix (PADM) with skin autograft.
Ten Japanese white rabbits were used as experimental animal, the dorsa of rabbits was symmetrically divided into four area, and then grouping was randomized. Full-thickness skin defects were created in Group A to Group C, and Group D was blank group with normal skin. The following procedures were performed: Group A: implanted with HA+PADM+ thin skin autografts, GroupB: implanted with PADM+ thin skin autografts and Group C: skin autografts, except for Group D. The wound tissue specimens were harvested at 70 days postoperatively, and they were recorded contracture rates of the grafts and the biomechanical compliance by Instron biomechanics tensiometer. The expression of collagens I and III in dermal fibroblasts of each group was determined by Western blot.
On postoperation day 70, the grafts contracture rates in Group A to Group C were 10.2% ± 0.6%, 36.6% ± 0.8%, 32.7% ± 1.4% respectively, there were notable significant difference among three groups (P = 0.000). Under the same tension, there was no significant difference in strain between Groups A and D ((83 ± 8) vs (81 ± 5) kPa, P = 0.552). Some significant difference in strain existed between Groups B and D ((215 ± 9) vs (81 ± 5) kPa, P = 0.000). The strain of Group C ((106 ± 7) kPa) was between Groups B and D, and significant strain difference existed among them (P = 0.000). The expressions of collagens I and III in Group A were higher than those in Group B, while the ratio of collagens I to III in Group A was lower than that in Group B.
Topical HA may be used to increase the expressions of type I and III collagen, decrease the ratio of collagen type I/III, and enhance the biomechanics performances of transplanted skin.
探讨在猪脱细胞真皮基质（PADM）与自体皮肤移植中添加透明质酸（HA）后对生物力学顺应性的影响。
选用10只日本大白兔作为实验动物，将兔背部对称分为4个区域，然后随机分组。A组至C组制造全层皮肤缺损，D组为正常皮肤的空白组。进行以下操作：A组：植入HA+PADM+薄自体皮；B组：植入PADM+薄自体皮；C组：仅植入自体皮，D组除外。术后70天采集伤口组织标本，用Instron生物力学张力仪记录移植物的挛缩率和生物力学顺应性。通过蛋白质免疫印迹法测定各组真皮成纤维细胞中I型和III型胶原蛋白的表达。
术后70天，A组至C组移植物挛缩率分别为10.2%±0.6%、36.6%±0.8%、32.7%±1.4%，三组间差异有统计学意义（P = 0.000）。在相同张力下，A组和D组的应变无显著差异（(83±8) vs (81±5) kPa，P = 0.552）。B组和D组的应变存在显著差异（(215±9) vs (81±5) kPa，P = 0.000）。C组的应变（(106±7) kPa）介于B组和D组之间，且它们之间存在显著的应变差异（P = 0.000）。A组中I型和III型胶原蛋白的表达高于B组，而A组中I型与III型胶原蛋白的比例低于B组。
局部应用HA可增加I型和III型胶原蛋白的表达，降低I/III型胶原蛋白的比例，并增强移植皮肤的生物力学性能。
