Mossakowski Medical Research Centre PAS, Laboratory of Respiratory Reflexes, Poland.
Eur J Pharmacol. 2011 Oct 1;668(1-2):257-63. doi: 10.1016/j.ejphar.2011.06.045. Epub 2011 Jul 8.
Anandamide (AEA), an endogenous cannabinoid and vanilloid receptor ligand, possesses anti-inflammatory properties. Transport of AEA through cytoplasm is facilitated by heat shock protein (HSP) Hsp70, which enhances the rate of cellular AEA uptake, possibly via direct interactions. In lungs, increased HSP expression is an endogenous, protective mechanism against acute lung inflammation. We hypothesised that AEA could enhance the expression of cytoprotective Hsp70 and Hsp25. Anaesthetised rats were injected intravenously with 1mg/kg AEA or saline. Lungs were removed 2 and 24 h after injection for evaluation of HSP expression. Hsp70 and Hsp25 expression in lungs was evaluated by immunohistochemistry. The relative levels of these HSPs in lung sections were determined through optical density measurements and western blotting of lung homogenates. Western blot and immunohistochemistry analyses indicated that expression of both proteins was significantly higher in AEA-injected animals than in control animals 2 and 24 h after treatment. AEA administration enhanced Hsp70 and Hsp25 expression in lungs. Therefore, AEA-HSP interactions could be involved in mechanisms protecting against lung inflammation, indicating a possible use of AEA as a treatment for lung inflammation.
内源性大麻素和香草素受体配体，即花生四烯酸乙醇胺（AEA），具有抗炎特性。热休克蛋白（HSP）Hsp70 可促进 AEA 通过细胞质的转运，从而提高细胞内 AEA 的摄取速度，其可能通过直接相互作用来实现。在肺部，HSP 表达增加是一种针对急性肺炎症的内源性保护机制。我们假设 AEA 可以增强细胞保护 HSP70 和 HSP25 的表达。给麻醉大鼠静脉注射 1mg/kg AEA 或生理盐水。在注射后 2 和 24 小时取出肺，用于评估 HSP 表达。通过免疫组织化学评估肺中 HSP70 和 HSP25 的表达。通过对肺匀浆的光密度测量和 Western blot 分析来确定这些 HSP 在肺组织切片中的相对水平。Western blot 和免疫组织化学分析表明，在治疗后 2 和 24 小时，AEA 处理组的两种蛋白质表达均明显高于对照组。AEA 给药可增强肺中 HSP70 和 HSP25 的表达。因此，AEA-HSP 相互作用可能参与了保护肺免受炎症的机制，这表明 AEA 可能可用于治疗肺部炎症。
