Chua C C, Chua B H
Department of Immunology and Microbiology, Wayne State University, School of Medicine, Detroit, Michigan 48202.
Connect Tissue Res. 1990;25(2):161-70. doi: 10.3109/03008209009006990.
We demonstrated previously that growth promoting factors in general could induce the secretion of interstitial collagenase into the medium of human fibroblast cells (HF). In this study, the effect of tumor necrosis factor-alpha (TNF-alpha) on the induction of collagenase and tissue inhibitor of metalloproteinases (TIMP) was examined. Stimulation of quiescent HF cells with 10 ng/ml TNF-alpha induced the secretion of Mr 57,000, 52,000 procollagenases into the medium. The collagenase activity was elevated 2.8-fold after TNF-alpha treatment. Northern blot analysis of the steady-state mRNA indicated a tenfold elevation of collagenase transcript after 24 h treatment with 10 ng/ml TNF-alpha. The increase in collagenase mRNA was due to transcriptional activation of collagenase gene activity. TIMP mRNA level increased three-fold after TNF-alpha treatment. The activity of TNF-alpha on collagenase and TIMP induction may play an important role in tissue inflammatory, repair and remodeling processes after wound and injury.
我们之前证明，一般而言，生长促进因子可诱导人成纤维细胞（HF）培养基中分泌间质胶原酶。在本研究中，检测了肿瘤坏死因子-α（TNF-α）对胶原酶和金属蛋白酶组织抑制剂（TIMP）诱导的影响。用10 ng/ml TNF-α刺激静止的HF细胞，可诱导分子量为57,000、52,000的前胶原酶分泌到培养基中。TNF-α处理后，胶原酶活性升高了2.8倍。对稳态mRNA的Northern印迹分析表明，用10 ng/ml TNF-α处理24小时后，胶原酶转录本升高了10倍。胶原酶mRNA的增加是由于胶原酶基因活性的转录激活。TNF-α处理后，TIMP mRNA水平增加了3倍。TNF-α对胶原酶和TIMP诱导的作用可能在伤口和损伤后的组织炎症、修复和重塑过程中起重要作用。
