Lambert C A, Lefebvre P Y, Nusgens B V, Lapière C M
Laboratory of Experimental Dermatology, University of Liège, CHU Sart Tilman, Belgium.
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):135-8. doi: 10.1042/bj2900135.
We have investigated the effect of electroporation on the expression of collagen alpha 1(I), collagenase, c-fos and c-jun genes in human dermal fibroblasts (HDF), human smooth muscle cells (HSMC) and HeLa cells. Collagenase and collagen mRNA levels were respectively increased and decreased in a voltage-dependent manner in HDF harvested 2 days after a sham electroporation. These effects were still observed 10 days after electroporation. Similar effects occurred in electroporated HSMC. Neither collagen nor collagenase mRNAs were detected in control or electroporated HeLa cells. c-fos and c-jun mRNA levels were also increased in electroporated HDF, HSMC and HeLa cells harvested 1 h after plating. This suggests that factor AP1 (fos/jun) could mediate the up-regulation of collagenase expression in electroporated HDF and HSMC. When electroporation of HDF was performed in the presence of H7, an inhibitor of protein kinase C, no increase in collagenase mRNA level was observed, suggesting that protein kinase C might be involved in the transduction of the effect. All the effects reported were also suppressed when cells were electroporated in a medium containing EGTA, suggesting that Ca2+ might mediate the transduction of this effect.
我们研究了电穿孔对人皮肤成纤维细胞（HDF）、人平滑肌细胞（HSMC）和HeLa细胞中Ⅰ型胶原α1、胶原酶、c-fos和c-jun基因表达的影响。在假电穿孔后2天收获的HDF中，胶原酶和胶原mRNA水平分别以电压依赖性方式升高和降低。电穿孔后10天仍观察到这些效应。电穿孔的HSMC中也出现了类似的效应。在对照或电穿孔的HeLa细胞中均未检测到胶原或胶原酶mRNA。在接种后1小时收获的电穿孔HDF、HSMC和HeLa细胞中，c-fos和c-jun mRNA水平也升高。这表明因子AP1（fos/jun）可能介导电穿孔HDF和HSMC中胶原酶表达的上调。当在蛋白激酶C抑制剂H7存在的情况下对HDF进行电穿孔时，未观察到胶原酶mRNA水平的增加，这表明蛋白激酶C可能参与了该效应的转导。当细胞在含有乙二醇双乙胺醚四乙酸（EGTA）的培养基中进行电穿孔时，所报道的所有效应也均被抑制，这表明Ca2+可能介导了该效应的转导。
