Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
J Colloid Interface Sci. 2011 Oct 1;362(1):100-6. doi: 10.1016/j.jcis.2011.06.039. Epub 2011 Jun 21.
In this study, the cooperative antibacterial efficiency of CdTe quantum dots (QDs) and rocephin against Escherichia coli (E. coli) was investigated. Colony-forming capability assay and diameter of inhibition zone (DIZ) measurement showed the antibiotic action of CdTe QDs-rocephin complex was better than the superposition of pure CdTe QDs and rocephin. The fractional inhibitory concentration index (FICI) indicated that CdTe QDs-rocephin complex could achieve great cooperative antimicrobial effects. The infrared ray (IR) spectrum, photoluminescence (PL) spectrophotometry, and detection of reactive oxygen species (ROS) indicated that CdTe QDs and rocephin formed a stable antimicrobial group through electrostatic attraction and hydrogen bonds and then killed the E. coli together. Meanwhile, the fluorescence intensity of CdTe QDs and the optical density (OD) value of E. coli showed a good linear relationship. Thus, dynamic monitoring to total bacterial concentration in the antibacterial process was realized by the CdTe QDs.
在这项研究中，研究了碲化镉量子点（QDs）和罗氏芬对大肠杆菌（E. coli）的协同抗菌效率。集落形成能力测定和抑菌圈直径（DIZ）测量表明，碲化镉量子点-罗氏芬复合物的抗生素作用优于纯碲化镉量子点和罗氏芬的叠加。部分抑菌浓度指数（FICI）表明，碲化镉量子点-罗氏芬复合物可以实现良好的协同抗菌效果。红外光谱、光致发光（PL）分光光度法和活性氧（ROS）检测表明，碲化镉量子点和罗氏芬通过静电吸引和氢键形成了稳定的抗菌基团，然后共同杀死大肠杆菌。同时，碲化镉量子点的荧光强度和大肠杆菌的光密度（OD）值呈良好的线性关系。因此，通过碲化镉量子点实现了对整个细菌浓度在抗菌过程中的动态监测。
