State Forensic Science Laboratory, Police line Campus, Tikrapara, Raipur, C.G., 492001, India.
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, C.G., 492010, India.
J Fluoresc. 2024 Mar;34(2):833-846. doi: 10.1007/s10895-023-03316-x. Epub 2023 Jun 30.
β-Lactum antibiotics are broad class of antibiotics which kills bacteria by inhibiting the formation of peptidoglycan that constitutes the bacterial cell wall. The resistance that develops in bacteria for antibiotics led the scientific world to think about the future aspects for modifying the way through which antibiotics are acted on the bacteria and become lethal for them. In this consequence, the potential of latest marketed antibiotics e.g. Amoxiciline (I), ceftazidim (II) have been evaluated after being conjugated with quantum dots. The surface of quantum dots has been conjugated with antibiotics by carbodiimide coupling with the help of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as conjugating agent between antibiotic and functionalized quantum dots. The antibacterial properties of QD-conjugated antibiotics have been determined by disc diffusion assay. The potency of QD-conjugated antibiotics has been estimated by determining their MIC for the selected strain of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Minimum inhibitory concentration study, minimum bactericidal concentration and growth pattern analysis revealed that QD-antibiotic conjugates showed slightly more prospective than pure native antibiotics against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria.
β-内酰胺类抗生素是一类广谱抗生素，通过抑制构成细菌细胞壁的肽聚糖的形成来杀死细菌。由于细菌对抗生素产生了耐药性，科学界开始思考如何改变抗生素作用于细菌的方式，使它们对细菌具有致命性。因此，对最新上市的抗生素（例如阿莫西林（I）、头孢他啶（II））进行了研究，在与量子点缀合后评估了它们的潜在应用。通过使用 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺（EDC）和 N-羟基琥珀酰亚胺（NHS）作为抗生素与功能化量子点之间的连接剂，通过碳二亚胺偶联将量子点表面与抗生素缀合。通过圆盘扩散试验测定了 QD-缀合抗生素的抗菌特性。通过测定所选革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌的最小抑菌浓度（MIC）来估计 QD-缀合抗生素的效价。最小抑菌浓度研究、最小杀菌浓度和生长模式分析表明，QD-抗生素缀合物对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌的效果略优于纯天然抗生素。
