Langford M P, Kaiwar R
Department of Ophthalmology, Louisiana State University Medical Center, Shreveport 71130.
J Virol Methods. 1990 Nov;30(2):151-60. doi: 10.1016/0166-0934(90)90016-9.
A spectrophotometric assay is described for the detection of interferon (IFN) dependent antibody (IDA) activity (i.e., antibodies that act with IFN to synergistically inhibit virus infection) and IFN independent antibody (IIA) activity (i.e., antibodies that act additively with IFN to inhibit virus infection). Four neutralizing monoclonal antibodies (mAb) against poliovirus type 1 (PV-1) were tested. Three mAb exhibited IDA activity and one mAb exhibited IIA activity against PV-1 strain Sabin. Concomitantly, the respective IDA and IIA activities were confirmed by a yield reduction assay. Also, the spectrophotometric assay detected IIA activity against PV-1 and IDA activity against PV-2 and PV-3 in human serum. Interestingly, antibody to a synthetic peptide of PV-1 capsid protein VP2 exhibited IIA activity against PV-2 strain MEF. Thus, this assay can facilitate the identification and investigation of IDA and IIA activities. Further, the assay adds economic feasibility to studying the natural occurrence of these antibody activities in the general population and can be useful in assessing the therapeutic potential of vaccine induced and hyperimmune antibodies.
本文描述了一种分光光度法，用于检测干扰素（IFN）依赖性抗体（IDA）活性（即与IFN协同作用以抑制病毒感染的抗体）和IFN非依赖性抗体（IIA）活性（即与IFN相加作用以抑制病毒感染的抗体）。对四种针对1型脊髓灰质炎病毒（PV-1）的中和单克隆抗体（mAb）进行了测试。三种mAb对PV-1 Sabin株表现出IDA活性，一种mAb表现出IIA活性。同时，通过产量降低试验证实了各自的IDA和IIA活性。此外，分光光度法检测到人类血清中针对PV-1的IIA活性以及针对PV-2和PV-3的IDA活性。有趣的是，针对PV-1衣壳蛋白VP2合成肽的抗体对PV-2 MEF株表现出IIA活性。因此，该试验有助于识别和研究IDA和IIA活性。此外，该试验为研究这些抗体活性在普通人群中的自然发生增加了经济可行性，并且可用于评估疫苗诱导抗体和超免疫抗体的治疗潜力。
