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1型脊髓灰质炎病毒结构多肽VP2上连续和不连续中和表位连接的分子基础

Molecular basis for linkage of a continuous and discontinuous neutralization epitope on the structural polypeptide VP2 of poliovirus type 1.

作者信息

Wiegers K J, Wetz K, Dernick R

机构信息

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Hamburg, Federal Republic of Germany.

出版信息

J Virol. 1990 Mar;64(3):1283-9. doi: 10.1128/JVI.64.3.1283-1289.1990.

DOI:10.1128/JVI.64.3.1283-1289.1990
PMID:1689392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249245/
Abstract

We obtained neutralizing monoclonal antibodies against a continuous neutralization epitope on VP2 of poliovirus type 1 strain Mahoney by using a combined in vivo-in vitro immunization procedure. The antibody-binding site was mapped to amino acid residues within the peptide segment (residues 164 through 170) of VP2 by competition with synthetic peptide and sequencing of resistant mutants. Cross-neutralization of these mutants with another neutralizing monoclonal antibody revealed a linkage of the continuous epitope and a discontinuous neutralization epitope involving both loops of the double-loop structure of VP2 at the twofold axis on the surface of the virion.

摘要

我们通过体内-体外联合免疫程序获得了针对脊髓灰质炎病毒1型Mahoney株VP2上一个连续中和表位的中和单克隆抗体。通过与合成肽竞争以及对耐药突变体进行测序，将抗体结合位点定位到VP2肽段（第164至170位氨基酸残基）内的氨基酸残基上。这些突变体与另一种中和单克隆抗体的交叉中和作用揭示了连续表位与一个不连续中和表位之间的联系，该不连续中和表位涉及病毒体表面二重轴处VP2双环结构的两个环。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0653/249245/868ef04c7235/jvirol00058-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0653/249245/868ef04c7235/jvirol00058-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0653/249245/868ef04c7235/jvirol00058-0332-a.jpg

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本文引用的文献

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