Department of Zoology, University of Pune, Ganeshkhind, Pune, India.
Islets. 2011 Sep-Oct;3(5):234-40. doi: 10.4161/isl.3.5.15941. Epub 2011 Sep 1.
Pancreatic islets are known to express low levels of antioxidant enzymes compared to other tissues and are therefore vulnerable to oxidative stress. Enhancing antioxidant defense mechanisms in pancreatic islets help them to cope better with oxidative stress. Persistent hyperglycemia under diabetic condition leads to continuous generation of reactive oxygen species, and different tissues exposed to this are oxidatively damaged depending on their antioxidant defense. Since islet cells are very poor in their antioxidant defense, our interest was to assess their antioxidant profile under normal, diabetic, insulin treated diabetic and untreated diabetic condition. On one hand, antioxidant defense was measured in terms of antioxidant enzymes and antioxidant molecules while on the other, damage caused to biomolecules was estimated. Our data demonstrate that oxidative damage to all biomolecules increased in islets cultured from diabetic animals, which enhanced further in islets from untreated diabetic animals. Insulin treatment significantly improved oxidative stress profile of islets indicating that the control of hyperglycemia leads to improvement in oxidative stress profile.
胰岛中的抗氧化酶水平通常比其他组织低，因此易受氧化应激的影响。增强胰岛中的抗氧化防御机制有助于其更好地应对氧化应激。在糖尿病状态下，持续的高血糖会导致活性氧的持续产生，不同组织暴露于这些活性氧中会根据其抗氧化防御能力受到氧化损伤。由于胰岛细胞的抗氧化防御能力非常差，我们的兴趣在于评估它们在正常、糖尿病、胰岛素治疗的糖尿病和未治疗的糖尿病状态下的抗氧化谱。一方面，抗氧化防御通过抗氧化酶和抗氧化分子来衡量，另一方面，生物分子的损伤程度也进行了评估。我们的数据表明，来自糖尿病动物的胰岛中所有生物分子的氧化损伤都增加了，未治疗的糖尿病动物的胰岛中的氧化损伤进一步增加。胰岛素治疗显著改善了胰岛的氧化应激谱，表明高血糖的控制可改善氧化应激谱。
