Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
Plant Signal Behav. 2011 Aug;6(8):1219-22. doi: 10.4161/psb.6.8.16181. Epub 2011 Aug 1.
Activation of NB-LRR-related UNI proteins by uni-1D mutation, a gain-of-function mutation of the UNI gene, induces some pathogenesis-related responses and also affects morphology through modulation of meristem activities. In a recent study we reported that the uni-1D phenotypes require cooperative action of ERECTA (ER) receptor kinase family members in UNI-expressing cells, suggesting that an intracellular signaling crosstalk between ER-family-dependent and UNI-triggered signaling pathways plays a significant role in the phenotypes. Further we recently succeeded in the establishment of a methodology for rapid identification of factors involved in the UNI function. EMS-induced causal mutations that suppress the uni-1D phenotypes could be identified using whole-genome-sequencing technologies with much less labor compared with the conventional map-based cloning method that is generally time-consuming and labor-intensive. Thus it would be now possible to intensively identify factors that play significant roles in regulation of UNI proteins and/or UNI-related signaling pathways.
uni-1D 突变（UNI 基因的功能获得性突变）激活 NB-LRR 相关 UNI 蛋白，诱导一些与发病机制相关的反应，并通过调节分生组织活性来影响形态。在最近的一项研究中，我们报告称，uni-1D 表型需要 UNI 表达细胞中 ERECTA（ER）受体激酶家族成员的协同作用，这表明 ER 家族依赖性和 UNI 触发的信号通路之间的细胞内信号串扰在表型中起着重要作用。此外，我们最近成功建立了一种快速鉴定 UNI 功能相关因子的方法。使用全基因组测序技术，可以比传统的基于图谱的克隆方法更快地鉴定出抑制 uni-1D 表型的 EMS 诱导的因果突变，与通常耗时耗力的图谱克隆方法相比，这种方法的工作量要小得多。因此，现在可以集中鉴定在 UNI 蛋白和/或 UNI 相关信号通路调节中起重要作用的因子。
