• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鉴定有丝分裂特异性p65二聚体作为人类M期促进因子的一个组成部分。

Identification of mitosis-specific p65 dimer as a component of human M phase-promoting factor.

作者信息

Meikrantz W, Suprynowicz F A, Halleck M S, Schlegel R A

机构信息

Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.

出版信息

Proc Natl Acad Sci U S A. 1990 Dec;87(24):9600-4. doi: 10.1073/pnas.87.24.9600.

DOI:10.1073/pnas.87.24.9600
PMID:2175907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC55220/
Abstract

Antisera raised against two mitosis-specific protein kinases from human cells recognized a single 65-kDa polypeptide (p65) that is present in similar amounts in interphase and mitotic cell extracts. Immunoblot analysis of reduced and unreduced extracts revealed that p65 exists as a 65-kDa monomer during interphase but forms a 130-kDa disulfide-linked homodimer during mitosis. Several different antibodies recognizing the p34cdc2 protein kinase and cyclin B components of M phase-promoting factor (MPF) coprecipitated p65 from mitotic but not from interphase extracts. In addition, an anti-p65 immunoaffinity column substantially depleted mitotic extracts of histon H1 kinase activity assayed under conditions diagnostic for MPF. These results suggest that active human MPF may be a complex of p34cdc2, cyclin B, and dimeric p65. A sulfhydryl cycle, proposed in the earlier literature on the biochemistry of mitosis, might underlie the dimerization of p65 and formation of active MPF.

摘要

针对来自人类细胞的两种有丝分裂特异性蛋白激酶产生的抗血清识别出一种单一的65 kDa多肽（p65），该多肽在间期和有丝分裂细胞提取物中的含量相似。对还原和未还原提取物的免疫印迹分析表明，p65在间期以65 kDa单体形式存在，但在有丝分裂期间形成130 kDa的二硫键连接的同源二聚体。几种识别M期促进因子（MPF）的p34cdc2蛋白激酶和细胞周期蛋白B成分的不同抗体，能从有丝分裂提取物而非间期提取物中共沉淀出p65。此外，在对MPF有诊断意义的条件下检测时，抗p65免疫亲和柱能使有丝分裂提取物中的组蛋白H1激酶活性大幅降低。这些结果表明，活性人类MPF可能是p34cdc2、细胞周期蛋白B和二聚体p65的复合物。早期关于有丝分裂生物化学的文献中提出的巯基循环，可能是p65二聚化和活性MPF形成的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/4c8d7363b6ab/pnas01049-0105-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/5b227d22433d/pnas01049-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/a7d6222a4ba5/pnas01049-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/8b2ad73ad8fe/pnas01049-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/7f92eec97540/pnas01049-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/4c8d7363b6ab/pnas01049-0105-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/5b227d22433d/pnas01049-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/a7d6222a4ba5/pnas01049-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/8b2ad73ad8fe/pnas01049-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/7f92eec97540/pnas01049-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d1f/55220/4c8d7363b6ab/pnas01049-0105-b.jpg

相似文献

1
Identification of mitosis-specific p65 dimer as a component of human M phase-promoting factor.鉴定有丝分裂特异性p65二聚体作为人类M期促进因子的一个组成部分。
Proc Natl Acad Sci U S A. 1990 Dec;87(24):9600-4. doi: 10.1073/pnas.87.24.9600.
2
Phorbol ester TPA rapidly prevents activation of p34cdc2 histone H1 kinase and concomitantly the transition from G2 phase to mitosis in synchronized HeLa cells.佛波酯TPA能迅速阻止p34cdc2组蛋白H1激酶的激活，并同时阻止同步化的HeLa细胞从G2期向有丝分裂的转变。
Exp Cell Res. 1994 Jun;212(2):383-8. doi: 10.1006/excr.1994.1158.
3
An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts.一种调节非洲爪蟾提取物中G2/M期转换的p34cdc2/细胞周期蛋白B抑制剂。
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):352-6. doi: 10.1073/pnas.93.1.352.
4
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.非洲爪蟾p34cdc2蛋白激酶在细胞周期中的去磷酸化与激活
Nature. 1989 Jun 22;339(6226):626-9. doi: 10.1038/339626a0.
5
M phase-promoting factor: its identification as the M phase-specific H1 histone kinase and its activation by dephosphorylation.
J Cell Sci Suppl. 1989;12:39-51. doi: 10.1242/jcs.1989.supplement_12.5.
6
A new screening test for antimitotic compounds using the universal M phase-specific protein kinase, p34cdc2/cyclin Bcdc13, affinity-immobilized on p13suc1-coated microtitration plates.一种使用固定在包被有p13suc1的微量滴定板上的通用M期特异性蛋白激酶p34cdc2/细胞周期蛋白Bcdc13的抗有丝分裂化合物筛选新试验。
Anticancer Res. 1991 Jul-Aug;11(4):1581-90.
7
Multiple forms of maturation-promoting factor in unfertilized Xenopus eggs.未受精的非洲爪蟾卵中成熟促进因子的多种形式。
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11530-4. doi: 10.1073/pnas.88.24.11530.
8
Kinetics of MPF and histone H1 kinase activity differ during the G2- to M-phase transition in mouse oocytes.在小鼠卵母细胞从G2期到M期的转变过程中，MPF和组蛋白H1激酶活性的动力学有所不同。
Int J Dev Biol. 1993 Dec;37(4):595-600.
9
Cyclin B2/cyclin-dependent kinase1 dissociation precedes CDK1 Thr-161 dephosphorylation upon M-phase promoting factor inactivation in Xenopus laevis cell-free extract.在非洲爪蟾无细胞提取物中，M期促进因子失活时，细胞周期蛋白B2/细胞周期蛋白依赖性激酶1解离先于细胞周期蛋白依赖性激酶1苏氨酸-161去磷酸化。
Int J Dev Biol. 2007;51(4):297-305. doi: 10.1387/ijdb.072292fc.
10
[From ovocyte to biochemistry of the cell cycle].[从卵母细胞到细胞周期的生物化学]
Verh K Acad Geneeskd Belg. 1991;53(4):365-85.

引用本文的文献

1
Dependence of the structure and mechanics of metaphase chromosomes on oxidized cysteines.中期染色体的结构和力学对氧化半胱氨酸的依赖性。
Chromosome Res. 2016 Sep;24(3):339-53. doi: 10.1007/s10577-016-9528-6. Epub 2016 May 5.
2
Activation of cyclin A-dependent protein kinases during apoptosis.细胞凋亡过程中细胞周期蛋白A依赖性蛋白激酶的激活。
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3754-8. doi: 10.1073/pnas.91.9.3754.
3
The crystal structure of p13suc1, a p34cdc2-interacting cell cycle control protein.p13suc1的晶体结构，一种与p34cdc2相互作用的细胞周期调控蛋白。

本文引用的文献

1
Studies on sulfhydryl groups during cell division of sea urchin egg. I. Glutatione.海胆卵细胞分裂过程中巯基的研究。I. 谷胱甘肽。
Exp Cell Res. 1959 Jan;16(1):24-41. doi: 10.1016/0014-4827(59)90192-2.
2
SH compounds in mitosis. I. The action of mercaptoethanol on the eggs of the sand dollar Dendraster excentricus.有丝分裂中的硫氢化合物。一、巯基乙醇对饼海胆（Dendraster excentricus）卵的作用。
Exp Cell Res. 1958 Jun;14(3):486-94. doi: 10.1016/0014-4827(58)90156-3.
3
Variations in sulfhydryl concentration during microsporocyte meiosis in the anthers of Lilium and Trillium.
EMBO J. 1995 Mar 1;14(5):1004-14. doi: 10.1002/j.1460-2075.1995.tb07081.x.
4
Crystal structure of the cell cycle-regulatory protein suc1 reveals a beta-hinge conformational switch.细胞周期调控蛋白suc1的晶体结构揭示了一种β-铰链构象开关。
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10232-6. doi: 10.1073/pnas.92.22.10232.
5
Chemically induced premature mitosis: differential response in rodent and human cells and the relationship to cyclin B synthesis and p34cdc2/cyclin B complex formation.化学诱导的早熟有丝分裂：啮齿动物和人类细胞的差异反应以及与细胞周期蛋白B合成和p34cdc2/细胞周期蛋白B复合物形成的关系。
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6843-7. doi: 10.1073/pnas.88.15.6843.
6
Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase.冈田酸模拟了细胞周期蛋白B-细胞分裂周期蛋白2激酶显微注射驱动海星卵母细胞进入M期所需的一种核成分。
J Cell Biol. 1991 Oct;115(2):337-44. doi: 10.1083/jcb.115.2.337.
百合和延龄草花药中小孢子母细胞减数分裂过程中巯基浓度的变化。
J Biophys Biochem Cytol. 1958 Mar 25;4(2):157-61. doi: 10.1083/jcb.4.2.157.
4
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.细胞周期蛋白：一种由海胆卵中母源mRNA所指定的蛋白质，在每次卵裂时都会被降解。
Cell. 1983 Jun;33(2):389-96. doi: 10.1016/0092-8674(83)90420-8.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.利用互补作用克隆了裂殖酵母细胞周期控制基因cdc2的一个人类同源基因。
Nature. 1987;327(6117):31-5. doi: 10.1038/327031a0.
7
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.裂殖酵母的细胞周期控制基因cdc2+编码一种可能受磷酸化调节的蛋白激酶。
Cell. 1986 Apr 25;45(2):261-8. doi: 10.1016/0092-8674(86)90390-9.
8
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.有丝分裂诱导剂nim1 +在控制有丝分裂起始的蛋白激酶同源物调控网络中发挥作用。
Cell. 1987 May 22;49(4):569-76. doi: 10.1016/0092-8674(87)90459-4.
9
Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.由细胞周期控制基因cdc2+的海星同源物编码的一种蛋白激酶在M期的激活。
Nature. 1988 Sep 15;335(6187):251-4. doi: 10.1038/335251a0.
10
Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2+ and suc1+.鉴定由cdc2+和suc1+编码的裂殖酵母细胞周期调节因子的人类同源物p34和p13。
Cell. 1987 Jul 17;50(2):319-25. doi: 10.1016/0092-8674(87)90227-3.