Kuykendall L David, Shao Jonathan Y
Molecular Plant Pathology Laboratory, Agricultural Research Service, US Department of Agriculture, Plant Sciences Institute, Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Building 004, Room 120, BARC-West, Beltsville, MD 20705, USA.
Int J Plant Genomics. 2011;2011:370548. doi: 10.1155/2011/370548. Epub 2011 Jun 7.
MAP3Kα, a gene that encodes a key conserved protein kinase, is responsible for initiating a rapid cascade of cellular events leading to localized cell death. Hypersensitive response, as it is termed, enables genetically resistant plants to limit microbial invasion under the right environmental conditions. Since knowledge of close physically linked genes is important for genome analysis and possibly for improving disease resistance, systematic DNA sequence analysis, gene annotation, and protein BLASTs were performed to identify and characterize genes in close physical proximity to a MAP3Kα-like gene in Beta vulgaris L. US H20. On the same 125 Kb BAC, callose synthase (BvCS) and phytochrome A (PhyA) genes were within 50 Kb of MAP3Kα. The close physical linkage of these genes may result from selection for coordinated responses to disease pressure. Bert, a new chromodomain-carrying gypsy-like LTR retrotransposon, resides within an intron of the BvCS gene, where it is transcribed from the opposing strand.
MAP3Kα是一个编码关键保守蛋白激酶的基因，负责启动一系列导致局部细胞死亡的快速细胞事件。这种被称为过敏反应的现象，能使具有遗传抗性的植物在适宜的环境条件下限制微生物入侵。由于了解紧密物理连锁的基因对于基因组分析以及可能对于提高抗病性很重要，因此进行了系统的DNA序列分析、基因注释和蛋白质BLAST，以鉴定和表征与甜菜US H20中一个类MAP3Kα基因紧密物理相邻的基因。在同一个125 Kb的细菌人工染色体（BAC）上，胼胝质合酶（BvCS）基因和光敏色素A（PhyA）基因位于MAP3Kα基因的50 Kb范围内。这些基因的紧密物理连锁可能是对疾病压力的协同反应选择的结果。Bert是一种携带新的染色质结构域的类吉普赛LTR反转录转座子，位于BvCS基因的一个内含子内，在那里它从相反的链转录。
