Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
PLoS One. 2011;6(7):e21802. doi: 10.1371/journal.pone.0021802. Epub 2011 Jul 8.
Wheat stripe rust is a destructive disease in the cool and humid wheat-growing areas of the world. Finding diverse sources of stripe rust resistance is critical for increasing genetic diversity of resistance for wheat breeding programs. Stripe rust resistance was identified in the alien species Psathyrostachys huashanica, and a wheat-P. huashanica amphiploid line (PHW-SA) with stripe rust resistance was reported previously. In this study, a P. huashanica 3Ns monosomic addition line (PW11) with superior resistance to stripe rust was developed, which was derived from the cross between PHW-SA and wheat J-11. We evaluated the alien introgressions PW11-2, PW11-5 and PW11-8 which were derived from line PW11 for reaction to new Pst race CYR32, and used molecular and cytogenetic tools to characterize these lines. The introgressions were remarkably resistant to CYR32, suggesting that the resistance to stripe rust of the introgressions thus was controlled by gene(s) located on P. huashanica chromosome 3Ns. All derived lines were cytologically stable in term of meiotic chromosome behavior. Two 3Ns chromosomes of P. huashanica were detected in the disomic addition line PW11-2. Chromosomes 1B of substitution line PW11-5 had been replaced by a pair of P. huashanica 3Ns chromosomes. In PW11-8, a small terminal segment from P. huashanica chromosome arm 3NsS was translocated to the terminal region of wheat chromosomes 3BL. Thus, this translocated chromosome is designated T3BL-3NsS. These conclusions were further confirmed by SSR analyses. Two 3Ns-specific markers Xgwm181 and Xgwm161 will be useful to rapidly identify and trace the translocated fragments. These introgressions, which had significant characteristics of resistance to stripe rust, could be utilized as novel germplasms for wheat breeding.
小麦条锈病是世界上凉爽潮湿的小麦种植区的一种破坏性疾病。寻找多样化的条锈病抗性来源对于增加小麦育种计划的抗性遗传多样性至关重要。在异源物种华山新麦草中发现了条锈病抗性，并且之前报道了具有条锈病抗性的小麦-华山新麦草双二倍体系（PHW-SA）。在这项研究中，开发了具有优异条锈病抗性的华山新麦草 3Ns 单体附加系（PW11），该系是通过 PHW-SA 和小麦 J-11 杂交得到的。我们评估了源自 PW11 的 3 条异源渐渗系 PW11-2、PW11-5 和 PW11-8 对新 Pst 小种 CYR32 的反应，并使用分子和细胞遗传学工具对这些系进行了表征。这些渐渗系对 CYR32 表现出显著的抗性，表明渐渗系对条锈病的抗性由位于华山新麦草染色体 3Ns 上的基因控制。所有衍生系在减数分裂染色体行为方面均表现出细胞学稳定性。在双体附加系 PW11-2 中检测到 2 条华山新麦草 3Ns 染色体。替换系 PW11-5 的 1BS 染色体已被一对华山新麦草 3Ns 染色体取代。在 PW11-8 中，来自华山新麦草染色体臂 3NsS 的一小段末端片段易位到小麦染色体 3BL 的末端区域。因此，这条易位染色体被命名为 T3BL-3NsS。这些结论通过 SSR 分析进一步得到证实。两个 3Ns 特异性标记 Xgwm181 和 Xgwm161 将有助于快速鉴定和追踪易位片段。这些具有显著条锈病抗性特征的渐渗系可作为小麦育种的新型种质资源加以利用。
