State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Theor Appl Genet. 2014 Apr;127(4):843-53. doi: 10.1007/s00122-014-2261-7. Epub 2014 Feb 2.
Stripe rust resistance transferred from Thinopyrum intermedium into common wheat was controlled by a single dominant gene, which mapped to chromosome 1B near Yr26 and was designated YrL693. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a highly destructive disease of wheat (Triticum aestivum). Stripe rust resistance was transferred from Thinopyrum intermedium to common wheat, and the resulting introgression line (L693) exhibited all-stage resistance to the widely virulent and predominant Chinese pathotypes CYR32 and CYR33 and to the new virulent pathotype V26. There was no cytological evidence that L693 had alien chromosomal segments from Th. intermedium. Genetic analysis of stripe rust resistance was performed by crossing L693 with the susceptible line L661. F(1), F(2), and F(2:3) populations from reciprocal crosses showed that resistance was controlled by a single dominant gene. A total 479 F(2:3) lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to six publicly available and three recently developed wheat genomic SSR markers. The linked markers were localized to wheat chromosome 1B using Chinese Spring nulli-tetrasomic lines, and the resistance gene was localized to chromosome 1B based on SSR and wheat genomic information. A high-density genetic map was also produced. The pedigree, molecular marker data, and resistance response indicated that the stripe rust resistance gene in L693 is a novel gene, which was temporarily designated YrL693. The SSR markers that co-segregate with this gene (Xbarc187-1B, Xbarc187-1B-1, Xgwm18-1B, and Xgwm11-1B) have potential application in marker-assisted breeding of wheat, and YrL693 will be useful for broadening the genetic basis of stripe rust resistance in wheat.
由中间偃麦草导入普通小麦的条锈病抗性由一个显性单基因控制，该基因位于 1B 染色体上，临近 Yr26，被命名为 YrL693。由条形柄锈菌（Puccinia striiformis f. sp. tritici，Pst）引起的条锈病是小麦（Triticum aestivum）的一种极具破坏性的疾病。将中间偃麦草的条锈病抗性导入普通小麦，所得的导入系（L693）对广泛流行且优势的中国生理小种 CYR32 和 CYR33 以及新的毒性生理小种 V26 表现出全生育期抗性。细胞学证据表明 L693 没有来自中间偃麦草的异源染色体片段。通过将 L693 与感病系 L661 杂交，对条锈病抗性进行了遗传分析。正反交 F1、F2 和 F2:3 群体的分析表明，抗性由一个显性单基因控制。共使用 479 个 F2:3 系和 781 对基因组简单重复序列（SSR）引物确定抗性基因的染色体定位。该基因与六个公开的和三个最近开发的小麦基因组 SSR 标记连锁。利用中国春缺体-四体系，将这些连锁标记定位到小麦 1B 染色体上，并根据 SSR 和小麦基因组信息，将该基因定位到 1B 染色体上。还生成了高密度遗传图谱。系谱、分子标记数据和抗性反应表明，L693 中的条锈病抗性基因是一个新基因，暂时命名为 YrL693。与该基因共分离的 SSR 标记（Xbarc187-1B、Xbarc187-1B-1、Xgwm18-1B 和 Xgwm11-1B）在小麦的分子标记辅助育种中具有潜在应用，而 YrL693 将有助于拓宽小麦条锈病抗性的遗传基础。
