Department of Chemistry and Biochemistry, Queens College of The City University of New York, Flushing, NY 11367, USA.
Chem Commun (Camb). 2011 Aug 28;47(32):9236-8. doi: 10.1039/c1cc12345a. Epub 2011 Jul 15.
The bioactivity of natural, long-chain ceramides has until now been studied after its delivery to cells in organic solvent mixtures containing dodecane. We have synthesized ceramides conjugated to a (6-bromo-7-hydroxycoumarin-4-yl)methyl group. The photocaged ceramide is efficiently released with 350 nm light in aqueous solution at neutral pH, thus providing a promising new tool to study ceramide's properties.
到目前为止，天然长链神经酰胺的生物活性一直是在其被递送到含有十二烷的有机溶剂混合物中的细胞中后进行研究的。我们已经合成了与（6-溴-7-羟基香豆素-4-基）甲基基团缀合的神经酰胺。在中性 pH 的水溶液中用 350nm 光可有效地释放光笼神经酰胺，从而为研究神经酰胺的性质提供了一种很有前途的新工具。
