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扩增核糖体DNA限制性分析作为评估毒物驱动的膨腹体(甲壳纲:等足目)后肠细菌种群变化的常规工具。

Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda).

作者信息

Santos Miguel J G, Sousa José Paulo, Tiago Igor, Veríssimo António, Lemos Marco F L

机构信息

CESAM and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.

出版信息

J Environ Monit. 2011 Aug;13(8):2102-4. doi: 10.1039/c1em10137d. Epub 2011 Jul 15.

Abstract

Changes in saprophytic organism's gut microbial communities may present a threat to organic matter breakdown which can ultimately lead to soil function impairment. In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.

摘要

腐生生物肠道微生物群落的变化可能对有机物分解构成威胁,最终导致土壤功能受损。在本研究中,扩增核糖体DNA限制性分析(ARDRA)被评估为一种潜在的简单分子工具,用于评估暴露于受污染食物的宽扁鼠妇后肠菌群结构的变化。在使用更具体、更复杂的方法之前,这种前瞻性工具也可用于各种目的和样本。

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