Vincent Center for Reproductive Biology, Vincent Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, Massachusetts, USA.
Dev Dyn. 2011 Aug;240(8):1929-37. doi: 10.1002/dvdy.22695.
We describe here a novel transgenic zebrafish, Tg(zpc:G4VP16/UAS:nfsB-mCherry) that effectively demonstrates the targeted oocyte ablation in the adult zebrafish ovary. This transgenic line expresses bacterial nitroreductase enzyme (nfsB) under the control of the oocyte-specific zona pellucida C (zpc) gene promoter. Adult transgenic females exposed to the prodrug metronidazole demonstrated near-complete ablation of growing oocytes, resulting in ovarian degeneration and complete cessation of reproductive function. Within 4 weeks of prodrug removal, treated fish demonstrated complete anatomical regeneration of the ovary and, within 7 weeks, ovarian function (fertility) was fully restored. Together, these results demonstrate functional renewal of the oocyte pool in the adult zebrafish ovary. Accordingly, this transgenic zebrafish model system provides a novel means to investigate ovarian growth dynamics in a genetically tractable vertebrate, and may be useful for evaluating signaling interactions that regulate gonadal development processes such as de novo oogenesis.
我们在这里描述了一种新型的转基因斑马鱼，Tg(zpc:G4VP16/UAS:nfsB-mCherry)，它可以有效地在成年斑马鱼的卵巢中靶向性地破坏卵母细胞。该转基因系在卵母细胞特异性透明带 C（zpc）基因启动子的控制下表达细菌硝基还原酶（nfsB）。暴露于前体药物甲硝唑的成年转基因雌性斑马鱼表现出生长卵母细胞的几乎完全破坏，导致卵巢退化和生殖功能完全停止。在前体药物去除后 4 周内，处理过的鱼表现出卵巢的完全解剖学再生，并且在 7 周内，卵巢功能（生育力）完全恢复。总之，这些结果表明成年斑马鱼卵巢中的卵母细胞库具有功能性更新。因此，这种转基因斑马鱼模型系统为在遗传上可操作的脊椎动物中研究卵巢生长动态提供了一种新方法，并且可能有助于评估调节性腺发育过程（如从头发生卵）的信号相互作用。
