Hou Entai, Wang Yingjuan, Li Duowei, Li Na, Chen Hongdong, Bu Huaiyu
College of Life Sciences, Northwest University, Xi'an 710069, China.
Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(7):823-7.
To study tissue culture of Vinca minor and determine the content of vincamine.
Leaf blades, stalks, root segment of V. minor were used as explants to study the effect of 2, 4-D,6-BA,NAA on its callus induction and vincamine contents in the orthogonal design experiment. In the peak period of callus formation, vincamine content in callus of V. minor and sterile plants was determined by HPLC. The experimental data was statistically analyzed.
The content of 6-BA and NAA had no significant effect on its callus induction. But the content of 2, 4-D had significant effect on its callus induction. Within 20,40,60 d, the content of vincamine in sterile plant was (0.015 +/- 0.003)%, (0.097 +/- 0.001)% , (0.113 +/- 0.06)%, respectively. In the peak period of callus formation, vincamine content in callus of leaf blades, stalks, root segment was (0.024 +/- 0.0025)%, (0.016 +/- 0.0015)%, (0.010 +/- 0.0015)%, respectively. To 30 days of subculture, vincamine content in callus of leaf blades, stalks, root segment was (0.041 +/- 0.002)%, (0.019 +/- 0001)%, (0.016 +/- 0.002)%, respectively.
The optimal hormone combination for callus initiation was MS +2, 4-D 1.0 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1). In different growth periods, vincamine content in sterile plants is significantly different. From different explants in callus vincamine content is different, in which leaves callus is significantly higher than that of stems, roots produced callus organization.
研究长春花的组织培养并测定长春胺含量。
以长春花的叶片、茎段、根段为外植体，采用正交设计实验研究2,4 - D、6 - BA、NAA对其愈伤组织诱导及长春胺含量的影响。在愈伤组织形成高峰期，采用高效液相色谱法测定长春花愈伤组织及无菌苗中长春胺的含量。对实验数据进行统计学分析。
6 - BA和NAA的含量对其愈伤组织诱导无显著影响。但2,4 - D的含量对其愈伤组织诱导有显著影响。在20、40、60 d时，无菌苗中长春胺含量分别为(0.015±0.003)%、(0.097±0.001)%、(0.113±0.06)%。在愈伤组织形成高峰期，叶片、茎段、根段愈伤组织中长春胺含量分别为(0.024±0.0025)%、(0.016±0.0015)%、(0.010±0.0015)%。继代培养30 d时，叶片、茎段、根段愈伤组织中长春胺含量分别为(0.041±0.002)%、(0.019±0.001)%、(0.016±0.002)%。
愈伤组织诱导的最佳激素组合为MS + 2,4 - D 1.0 mg·L⁻¹ + 6 - BA 0.5 mg·L⁻¹ + NAA 0.5 mg·L⁻¹。在不同生长时期，无菌苗中长春胺含量差异显著。不同外植体形成的愈伤组织中长春胺含量不同，其中叶片愈伤组织中长春胺含量显著高于茎、根产生的愈伤组织。
