Department of Biochemistry, Faculty of Medicine, Ovidius University of Constanta, Street Universitatii, No. 1, 900470 Constanta, Romania.
Department of Chemistry and Chemical Engineering, Ovidius University of Constanta, 124 Mamaia Blvd., 900527 Constanta, Romania.
Molecules. 2023 Jul 26;28(15):5667. doi: 10.3390/molecules28155667.
In recent decades, new alternative therapies using drugs containing active ingredients of natural origin have been a hot topic for medical research. Based on the confirmed therapeutic potential of the plant, considered in the specialized literature to be of pharmaceutical interest, the purpose of this study is to determine the chemical and mineral composition of the plant grown in the Dobrogea area, with a view to its use in the formulation of dermal preparations. For this purpose, plant materials were collected from the mentioned area and hydroalcoholic macerates of different concentrations were obtained: 40%, 70% and 96% from leaves (F40, F70, F96) and stems (T40, T70, T96) of plant to determine the optimal extraction solvent. The hydroalcoholic macerates were analyzed via the HPLC method for the identification and quantification of the main bioactive compounds, and two methods were used to evaluate their antioxidant properties: the DPPH radical scavenging test and the photochemiluminescence method. HPLC analysis showed the presence of four indole alkaloids: vincamine, 1,2-dehydroaspidospermidine, vincaminoreine and eburnamonine. Vincamine was the alkaloid found in the highest concentration in Vinca leaves (2.459 ± 0.035 mg/100 g d.w.). The antioxidant activity of hydroalcoholic macerates showed values between 737.626-1123.500 mg GAE/100 g d.w (DPPH test) and 77.439-187.817 mg TE/100 g d.w (photochemiluminescence method). The concentrations of toxic metals Cd, Cu, Ni, Pb in dried leaves and stems of , determined by AAS, were below detection limits.
近几十年来，使用含有天然来源活性成分的药物的新替代疗法一直是医学研究的热门话题。基于该植物被认为具有治疗潜力，且在专业文献中被认为具有药用价值，本研究旨在确定在多布罗加地区生长的 植物的化学和矿物质组成，以期将其用于配制皮肤制剂。为此，从该地区采集了植物材料，并获得了不同浓度的水醇提取物：40%、70%和 96%的叶片（F40、F70、F96）和茎（T40、T70、T96），以确定最佳提取溶剂。通过 HPLC 法分析水醇提取物，以鉴定和定量主要生物活性化合物，并使用两种方法评估其抗氧化性能：DPPH 自由基清除试验和光化学发光法。HPLC 分析表明存在四种吲哚生物碱：长春胺、1,2-脱氢aspermidine、长春胺和 eburnamonine。长春胺是在长春叶中发现的浓度最高的生物碱（2.459 ± 0.035 mg/100 g d.w.）。水醇提取物的抗氧化活性值在 737.626-1123.500 mg GAE/100 g d.w（DPPH 试验）和 77.439-187.817 mg TE/100 g d.w（光化学发光法）之间。通过 AAS 测定，干燥的 和茎中的有毒金属 Cd、Cu、Ni、Pb 的浓度均低于检测限。
