CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
J Agric Food Chem. 2011 Sep 14;59(17):9386-93. doi: 10.1021/jf201801q. Epub 2011 Aug 3.
High-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS(n)) were used to investigate the phenolic constituents in methanol, water, and methanol/water extracts of Eucalyptus globulus Labill. bark. Twenty-nine phenolic compounds were identified, 16 of them referenced for the first time as constituents of E. globulus bark, namely, quinic, dihydroxyphenylacetic, and caffeic acids, bis(hexahydroxydiphenoyl (HHDP))-glucose, galloyl-bis(HHDP)-glucose, galloyl-HHDP-glucose, isorhamentin-hexoside, quercetin-hexoside, methylellagic acid (EA)-pentose conjugate, myricetin-rhamnoside, isorhamnetin-rhamnoside, mearnsetin, phloridzin, mearnsetin-hexoside, luteolin, and a proanthocyanidin B-type dimer. Digalloylglucose was identified as the major compound in the methanol and methanol/water extracts, followed by isorhamnetin-rhamnoside in the methanol extract and by catechin in the methanol/water extract, whereas in the water extract catechin and galloyl- HHDP-glucose were identified as the predominant components. The methanol/water extract was shown be the most efficient to isolate phenolic compounds identified in E. globulus bark.
高效液相色谱-电喷雾电离质谱（HPLC-ESI-MS）和串联质谱（MS(n)）被用于研究蓝桉树皮甲醇、水和甲醇/水提取物中的酚类成分。共鉴定出 29 种酚类化合物，其中 16 种为蓝桉树皮的成分，首次被鉴定为奎尼酸、二羟苯乙酸和咖啡酸、双（六氢二苯酰基（HHDP））-葡萄糖、没食子酰基-双（HHDP）-葡萄糖、没食子酰基-HHDP-葡萄糖、异荭草素-己糖苷、槲皮素-己糖苷、甲基鞣花酸（EA）-戊糖缀合物、杨梅素-鼠李糖苷、异鼠李素-鼠李糖苷、山奈酚、根皮苷、山奈酚-己糖苷、木樨草素和原花青素 B 型二聚体。在甲醇和甲醇/水提取物中，双没食子酰基葡萄糖是主要化合物，其次是甲醇提取物中的异鼠李素-鼠李糖苷和甲醇/水提取物中的儿茶素，而在水提取物中，儿茶素和没食子酰基-HHDP-葡萄糖是主要成分。甲醇/水提取物是分离蓝桉树皮中鉴定出的酚类化合物最有效的方法。
