Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland.
J Agric Food Chem. 2012 Sep 5;60(35):8672-83. doi: 10.1021/jf302925v. Epub 2012 Aug 22.
Phenolic compounds were extracted from dried emblic leafflower (Phyllanthus emblica L.) fruits with methanol and separated by Sephadex LH-20 column chromatography. The raw extracts and fractions were analyzed with HPLC coupled with diode array UV spectroscopy, electrospray ionization mass spectrometry, and tandem mass spectrometry. Mucic acid gallate, mucic acid lactone gallate, monogalloylglucose, gallic acid, digalloylglucose, putranjivain A, galloyl-HHDP-glucose, elaeocarpusin, and chebulagic acid were suggested to be the most abundant compounds in the crude methanol extracts of the fruits. In addition, 144 peaks were detected, of which 67 were tentatively identified mostly as ellagitannins, flavonoids, and simple gallic acid derivatives in the fractions. The results indicated the presence of neochebulagic acid, isomers of neochebuloyl galloylglucose, chebuloyl neochebuloyl galloylglucose, ellagic acid glycosides, quercetin glycosides, and eriodictyol coumaroyl glycosides in the fruits. The study provides a systematic report of the retention data and characteristics of UV, MS, and MS/MS spectra of the phenolic compounds in the fruits of emblic leafflower. The fruits of two varieties (Ping Dan No 1 and Fruity) from Guangxi Province differed from those of wild Tian Chuan emblic leafflower from Fujian Province in the content and profile of phenolic compounds.
从干的余甘子（Phyllanthus emblica L.）果实中用甲醇提取酚类化合物，并通过 Sephadex LH-20 柱层析进行分离。用高效液相色谱法与二极管阵列紫外光谱、电喷雾电离质谱和串联质谱联用分析原始提取物和馏分。推测没食子酸奎宁酸酯、没食子酸内酯奎宁酸酯、单没食子酰葡萄糖、没食子酸、二没食子酰葡萄糖、putranjivain A、没食子酰 HHDP-葡萄糖、elaeocarpusin 和 chebulagic 酸是果实粗甲醇提取物中含量最丰富的化合物。此外，检测到 144 个峰，其中 67 个在馏分中被初步鉴定为主要为鞣花单宁、类黄酮和简单没食子酸衍生物。结果表明，果实中存在新诃子酸、新诃子酰基葡萄糖的异构体、诃子酰基新诃子酰基葡萄糖、鞣花酸糖苷、槲皮苷和橙皮素 coumaroyl 糖苷。该研究提供了余甘子果实中酚类化合物的保留数据和 UV、MS 和 MS/MS 光谱特征的系统报告。来自广西的两个品种（平单 1 号和富果）的果实与来自福建的野生甜川余甘子的果实在酚类化合物的含量和谱型上有所不同。
