Ozer Hamza, Taşkesen Anıl, Kul Oğuz, Selek Hakan Y, Turanlı Sacit, Köse Kenan
Department of Orthopedics and Traumatology, Medicine Faculty of Gazi University, Ankara, Turkey.
Eklem Hastalik Cerrahisi. 2011 Aug;22(2):100-6.
We evaluated the effects of glucosamine chondroitine sulphate (glcN-CS) on rat Achilles tendons in this experimental animal study.
Thirty Wistar albino type rats weighing 300-350 g were randomly grouped into two. Group A: Achilles tendons were tenotomized, repaired and, glcN-CS given orally. Group B: Achilles tendons were tenotomized and repaired. All the rats were fed with pellet chow. Five rats in each group were sacrificed at 4, 8, and 12 weeks interval. Tensile strength of three tendon specimen from each group were tested biomechanically and two specimen were analysed histopathologically via immunohistochemical staining and hematoxylin and eosin staining. Contralateral side Achilles tendons were also grouped and analyzed as controls (group C and D).
Histological results showed well organized collagen formation and less inflammation in group A. Biomechanical testing showed higher tendon strength in group A at eight weeks which is not statisticaly significant (55.3±4.84N, p=0.078). Rats which were were given glcN-CS had greater tendon strength which is statisticaly significant (50.01±5.62, p=0.014).
Glucoseamine chondroitine sulphate improved results of Achilles tendon healing in rats. This result might be due to decreased inflammation and stimulation of collagen synthesis. Although glcN-CS is recommended only in osteoarthritic patients to relieve symptoms and signs it can also be prefered in treatment of soft tissue injuries formed during sports activities.
在本实验动物研究中，我们评估了硫酸氨基葡萄糖软骨素（glcN-CS）对大鼠跟腱的影响。
将30只体重300 - 350克的Wistar白化大鼠随机分为两组。A组：切断并修复跟腱，口服glcN-CS。B组：切断并修复跟腱。所有大鼠均喂食颗粒饲料。每组分别在4周、8周和12周时处死5只大鼠。对每组的三个肌腱标本进行生物力学测试，对两个标本通过免疫组织化学染色和苏木精-伊红染色进行组织病理学分析。对侧跟腱也分组并作为对照进行分析（C组和D组）。
组织学结果显示A组胶原形成良好且炎症较少。生物力学测试显示A组在8周时肌腱强度较高，但无统计学意义（55.3±4.84N，p = 0.078）。给予glcN-CS的大鼠肌腱强度更高，具有统计学意义（50.01±5.62，p = 0.014）。
硫酸氨基葡萄糖软骨素改善了大鼠跟腱愈合的结果。这一结果可能是由于炎症减少和胶原合成受到刺激。尽管glcN-CS仅被推荐用于骨关节炎患者以缓解症状和体征，但它也可用于治疗体育活动中形成的软组织损伤。
