Lum L G, Burns E, Janson M M, Martin P J, Giddings B R, Seigneuret M C, Noges J E, Galoforo S C
Department of Medicine, Wayne State University, Detroit, Michigan.
J Clin Immunol. 1990 Sep;10(5):255-64. doi: 10.1007/BF00916701.
This investigation uses a system for inducing and detecting anti-tetanus toxoid antibody (anti-TT) synthesis to study specific antibody (Ab) synthesis by bone marrow mononuclear cells (MC). We measured the amounts of anti-TT secreted and the number of B cells secreting antibody (Ab). The ELISA plaque detects single B cells secreting specific Ab. The results show that (1) spontaneous anti-TT secretion by MC is higher than spontaneous anti-TT secretion by peripheral blood lymphocytes (PBL) using an ELISA plaque (P less than 0.01); (2) spontaneous anti-TT production by MC correlated with the serum anti-TT titers as measured by an ELISA (r = 0.75, P = 0.005); (3) two types of marrow B cells were identified--one that spontaneously secretes anti-TT and another that produces anti-TT after TT-stimulation; (4) the frequency of anti-TT-secreting B cells is higher in MC than in PBL; (5) the amount of Ab secreted per marrow B cell is not different from that secreted by a peripheral B cell; and (6) marrow B cells could be induced to produce anti-TT in vitro up to 10 months without added cytokines. These results show that bone marrow is a major repository for differentiated B cells that spontaneously produce Abs to maintain circulating Abs titers and for memory B cells that can be induced to produce specific Ab.
本研究采用一种诱导和检测抗破伤风类毒素抗体（抗-TT）合成的系统，以研究骨髓单个核细胞（MC）合成特异性抗体（Ab）的情况。我们测定了分泌的抗-TT量以及分泌抗体（Ab）的B细胞数量。ELISA蚀斑检测分泌特异性Ab的单个B细胞。结果表明：（1）使用ELISA蚀斑法，MC的自发抗-TT分泌高于外周血淋巴细胞（PBL）的自发抗-TT分泌（P<0.01）；（2）MC的自发抗-TT产生与ELISA测定的血清抗-TT滴度相关（r = 0.75，P = 0.005）；（3）鉴定出两种骨髓B细胞——一种自发分泌抗-TT，另一种在TT刺激后产生抗-TT；（4）MC中分泌抗-TT的B细胞频率高于PBL；（5）每个骨髓B细胞分泌的Ab量与外周B细胞分泌的量无差异；（6）在不添加细胞因子的情况下，骨髓B细胞在体外可被诱导产生抗-TT长达10个月。这些结果表明，骨髓是分化B细胞的主要储存库，这些B细胞可自发产生抗体以维持循环抗体滴度，也是记忆B细胞的主要储存库，记忆B细胞可被诱导产生特异性抗体。
