CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisboa, Portugal.
Helicobacter. 2011 Aug;16(4):320-6. doi: 10.1111/j.1523-5378.2011.00847.x.
Animal models have been widely used to study Helicobacter pylori infection. Evaluation of H. pylori infection status following experimental inoculation of mice usually requires euthanasia. The (13) C-urea breath test ((13) C-UBT) is both sensitive and specific for detection of H. pylori in humans. Thus, it would be very useful to have such a test with the same accuracy for the follow-up of this infection in animal models of gastric infection. Accordingly, the purpose of this study was to develop and evaluate a (13) C-UBT method for following the course of H. pylori infection in a mouse model.
A total of 50 female C57BL/6 mice were gavaged three times with either 10(8) colony-forming units of H. pylori (n=29) or saline solution only (n=21). After 2 months of infection, mice were fasted for 14 hours and (13) C-UBT was performed using 300 μg of (13) C-urea. The mice were killed, and the stomach was removed and processed for immunohistochemistry and PCR.
The optimal time for breath sample collection in mice was found to be 15 minutes. The (13) C-UBT cutoff was set at 3.0‰ δPDB. Using PCR as the gold standard, the sensitivity of (13) C-UBT and immunohistochemistry was 96.6 and 72.4%, respectively, while the specificity was 85.7 and 95.2%, respectively.
(13) C-UBT was shown to be a reliable method for the detection of H. pylori infection in C57BL/6 mice and was even more accurate than immunohistochemistry. The use of (13) C-UBT in the mouse model of H. pylori infection can be very useful to detect the bacterium without the need to kill the animals in long-term time course studies.
动物模型已被广泛用于研究幽门螺杆菌感染。在对小鼠进行实验接种后，评估 H. pylori 感染状态通常需要安乐死。(13)C-尿素呼气试验((13)C-UBT) 对人类 H. pylori 的检测具有高度的敏感性和特异性。因此，如果有一种具有相同准确性的检测方法可以用于动物胃感染模型中这种感染的后续检测，将会非常有用。因此，本研究旨在开发和评估一种用于监测小鼠模型中 H. pylori 感染过程的 (13)C-UBT 方法。
共 50 只雌性 C57BL/6 小鼠，用 10(8)个集落形成单位的 H. pylori(29 只)或仅生理盐水(21 只)进行三次灌胃。感染 2 个月后，小鼠禁食 14 小时，用 300μg(13)C-尿素进行 (13)C-UBT。处死小鼠，取出胃进行免疫组织化学和 PCR 检测。
发现小鼠最佳呼气样本采集时间为 15 分钟。(13)C-UBT 截断值设定为 3.0‰ δPDB。以 PCR 为金标准，(13)C-UBT 和免疫组织化学的敏感性分别为 96.6%和 72.4%，特异性分别为 85.7%和 95.2%。
(13)C-UBT 被证明是一种可靠的检测 C57BL/6 小鼠 H. pylori 感染的方法，甚至比免疫组织化学更准确。在 H. pylori 感染的小鼠模型中使用 (13)C-UBT 可以非常有用，无需在长期时间过程研究中杀死动物即可检测细菌。
