Department of Oncology, The People's Hospital of Taizhou, Taizhou, China.
Dis Esophagus. 2012 Feb;25(2):121-9. doi: 10.1111/j.1442-2050.2011.01219.x. Epub 2011 Jul 15.
The aim of this study was to examine the expression of the molecular markers cyclooxygenase-2 (COX-2), Ki-67, cyclin A, and p27 in patients with esophageal squamous cell carcinoma (ESCC), to ascertain the relationship of these makers with the clinicopathological significance of the patients, and to assess the additional prognostic value of the expression profile of these proteins for ESCC patients. The expression levels of COX-2, Ki-67, cyclin A, and p27 proteins of a series of primarily resected ESCC samples were determined by immunohistochemistry method. Clinicopathological and molecular factors affecting survival were analyzed by multivariate analysis. A total of 78 specimens were included in this study. Expression of COX-2 was observed in 43 (55.1%) cases, and high levels of expression of Ki-67, p27, and cyclin A were observed in 57 (73.0%), 33 (42.3%), 43 (55.1%) cases, respectively. The results of univariate survival analysis indicated that more advanced tumor stage, lymph node involvement, systemic dissemination, the levels of expression of COX-2, Ki-67, cyclin A, and p27 were associated with survival (all P-value < 0.05). Multifactorial survival analysis revealed that only lymph node involvement, over-expression of cyclin A, and low p27 expression were associated with the survival of the patients (hazard ratios = 2.83, 4.7, 2.9, respectively; P= 0.025, 0.042, 0.005, respectively). Among the molecular markers assessed, the expression of cell proliferation markers cyclin A and p27 are independent prognostic factors in patients with ESCC, whereas neither COX-2 nor Ki-67 is of independent prognostic value.
本研究旨在检测环氧化酶-2（COX-2）、Ki-67、细胞周期蛋白 A 和 p27 等分子标志物在食管鳞状细胞癌（ESCC）患者中的表达情况，确定这些标志物与患者临床病理特征的关系，并评估这些蛋白表达谱对 ESCC 患者的预后价值。采用免疫组化法检测一系列原发性 ESCC 标本中 COX-2、Ki-67、细胞周期蛋白 A 和 p27 蛋白的表达水平，采用多因素分析方法分析影响生存的临床病理和分子因素。本研究共纳入 78 例标本。43 例（55.1%）观察到 COX-2 表达，57 例（73.0%）、33 例（42.3%）、43 例（55.1%）患者 Ki-67、p27、细胞周期蛋白 A 表达水平较高。单因素生存分析结果表明，肿瘤分期较晚、淋巴结转移、全身转移、COX-2、Ki-67、细胞周期蛋白 A 和 p27 表达水平与生存有关（均 P 值<0.05）。多因素生存分析显示，只有淋巴结转移、细胞周期蛋白 A 过表达和 p27 低表达与患者的生存有关（危险比分别为 2.83、4.7、2.9；P 值分别为 0.025、0.042、0.005）。在评估的分子标志物中，细胞增殖标志物细胞周期蛋白 A 和 p27 的表达是 ESCC 患者独立的预后因素，而 COX-2 和 Ki-67 均无独立的预后价值。
