Huang Jun-Xing, Yao Juan, Lin Mao-Song, Lin Mei, Xiao Wei, Yu Hong, Chen Ping, Qian Rong-Yu
Department of Oncology, The People's Hospital of Taizhou, Taizhou Medical School, Nantong & Jiangsu University Taizhou 225300, Jiangsu, P. R. China.
Department of Pathology, The People's Hospital of Taizhou, Taizhou Medical School, Nantong & Yangzhou University Taizhou 225300, Jiangsu, P. R. China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15920-9. eCollection 2015.
This study aims to ascertain the relationship of tumor metastasis-associated markers cyclin D1, connective tissue growth factor (CTGF) and vascular endothelial growth factor (VEGF) with the clinicopathologic features and prognosis of patients with esophageal squamous cell carcinoma (ESCC), and to investigate their value in ESCC molecular classification. The expression of cyclin D1, CTGF and VEGF in 100 specimens from patients and 20 from normal esophageal mucosa were detected by immunohistochemistry. The relationship of their expression with prognosis of the patients with ESCC was evaluated by Cox regression model and Kaplan-Meier survival curve analysis. High levels of expression of cyclin D1, CTGF, and VEGF were observed in 61 (61%), 53 (53%), 49 (49%) cases, respectively. Univariate survival analysis indicated that the levels of expression of cyclin D1, CTGF and VEGF were associated with survival (all P-value < 0.05). Multivariate analysis indicated that cyclin D1 and VEGF were independent prognostic factors affecting the three-year survival rate of patients (P = 0.001, 0.017, respectively). Furthermore, high level expression of cyclin D1, CTGF and VEGF in stage I patients was found associated with poor three-year survival rate (all P-value < 0.05). The prognosis probably was favorable for patients with low expression of cyclin D1 even in stage III, or VEGF even in stage IV. Tumor metastasis-associated markers such as cyclin D1 and VEGF may be independent prognostic factors affecting survival rate of postoperative ESCC patients. It is possible to judge prognosis better and tailor treatments to each individual patient when these markers were applied to ESCC molecular classification.
本研究旨在确定肿瘤转移相关标志物细胞周期蛋白D1、结缔组织生长因子（CTGF）和血管内皮生长因子（VEGF）与食管鳞状细胞癌（ESCC）患者临床病理特征及预后的关系，并探讨它们在ESCC分子分类中的价值。采用免疫组织化学法检测100例ESCC患者标本及20例正常食管黏膜标本中细胞周期蛋白D1、CTGF和VEGF的表达。通过Cox回归模型和Kaplan-Meier生存曲线分析评估它们的表达与ESCC患者预后的关系。结果显示，细胞周期蛋白D1、CTGF和VEGF高表达分别见于61例（61%）、53例（53%）、49例（49%）。单因素生存分析表明，细胞周期蛋白D1、CTGF和VEGF的表达水平与生存相关（所有P值<0.05）。多因素分析表明，细胞周期蛋白D1和VEGF是影响患者三年生存率的独立预后因素（P值分别为0.001、0.017）。此外，发现I期患者中细胞周期蛋白D1、CTGF和VEGF高表达与三年生存率低相关（所有P值<0.05）。即使在III期，细胞周期蛋白D1低表达的患者，或在IV期，VEGF低表达的患者，预后可能较好。细胞周期蛋白D1和VEGF等肿瘤转移相关标志物可能是影响ESCC术后患者生存率的独立预后因素。将这些标志物应用于ESCC分子分类时，有可能更好地判断预后并为每个患者制定个性化治疗方案。
