用于快速且廉价的HLA - DQB1基因分型的多重连接依赖探针扩增技术
Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.
作者信息
Akers N K, Curry J D, Smith M T, Bracci P M, Skibola C F
机构信息
Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, Berkeley, CA, USA.
出版信息
Tissue Antigens. 2011 Oct;78(4):275-80. doi: 10.1111/j.1399-0039.2011.01737.x. Epub 2011 Jul 18.
Abstract
Many effective options exist to accurately type DNA for human leukocyte antigen (HLA) alleles. However, most of the existing methods are excessively costly in terms of overall monetary costs, DNA requirements, and proprietary software. We present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at two digits, with even greater specificity for the HLA-DQB1*06 allele family, by using the multiplexed ligation-dependent probe amplification technology. This assay provides more specific allele data than genome-wide analysis and is more affordable than sequencing, making it a useful intermediate for researchers seeking to accurately allelotype human DNA samples.
摘要
有许多有效的方法可用于准确鉴定人类白细胞抗原（HLA）等位基因的DNA类型。然而，就总体金钱成本、DNA需求量和专有软件而言，现有的大多数方法成本过高。我们提出了一种新型检测方法，通过使用多重连接依赖探针扩增技术，能够以两位数字解析杂合的HLA - DQB1等位基因型，对HLA - DQB1*06等位基因家族具有更高的特异性。该检测方法比全基因组分析提供更特异的等位基因数据，且比测序更经济实惠，使其成为寻求准确鉴定人类DNA样本等位基因型的研究人员的有用中间手段。
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