School of Public Health, University of California, Berkeley, CA, USA.
Leuk Lymphoma. 2011 Jan;52(1):53-8. doi: 10.3109/10428194.2010.532888. Epub 2010 Dec 6.
In a recent genome-wide association study of follicular lymphoma (FL), we identified novel risk alleles on chromosome 6p21.33 that appeared to be part of an extended haplotype including HLA-DRB10101, DQA10101, and DQB10501. To follow up on these findings, we obtained 2-4 digit HLA-DQB1 allelotypes on a subset of 265 cases of FL and 757 controls using a novel assay that applies multiplexed ligation-dependent probe amplification (MLPA). We confirmed a positive association between FL and the HLA-DQB105 allele group (OR = 1.70, 95% CI 1.28-2.27; adjusted p-value = 0.013) and also identified an allele group inversely associated with FL risk, HLA-DQB1*06 (OR = 0.51, 95% CI 0.38-0.69; adjusted p-value = 4.46 × 10(-5)). Although these findings require verification, the role of HLA class II proteins in B-cell survival and proliferation makes this a biologically plausible association.
在最近一项关于滤泡性淋巴瘤（FL）的全基因组关联研究中，我们在染色体 6p21.33 上发现了新的风险等位基因，这些基因似乎是包含 HLA-DRB10101、DQA10101 和 DQB10501 的扩展单倍型的一部分。为了跟进这些发现，我们使用一种新的应用多重连接依赖性探针扩增（MLPA）的检测方法，在 265 例 FL 病例和 757 例对照中获得了 HLA-DQB1 2-4 位等位基因。我们证实了 HLA-DQB105 等位基因组与 FL 之间存在正相关（OR=1.70，95%CI 1.28-2.27；调整后的 p 值=0.013），并且还确定了一个与 FL 风险呈负相关的等位基因组 HLA-DQB1*06（OR=0.51，95%CI 0.38-0.69；调整后的 p 值=4.46×10(-5)）。尽管这些发现需要进一步验证，但 HLA Ⅱ类蛋白在 B 细胞存活和增殖中的作用使得这一关联具有生物学意义。
