Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al Ahsa, Kingdom of Saudi Arabia.
Theriogenology. 2011 Oct 1;76(6):1126-33. doi: 10.1016/j.theriogenology.2011.05.021. Epub 2011 Jul 16.
Testicles were isolated from thirty five apparently healthy dromedary camels (Camelus dromedarius), aged between 5 to 18 years, in a local slaughterhouse during the rutting season. Epididymal fluid was collected from one epididymis for determination of twelve biochemical and antioxidant parameters using ELISA commercial kits. Spermatozoa were harvested from each region of the other epididymis (head, body and tail) and stored in SHOTOR®, Green buffer® + 20% egg yolk and INRA-96® extenders at 5 and 30 °C. Results revealed that, in the epididymal fluid, concentrations of testosterone, glucose, albumin, total protein, cholesterol, fatty acids, iron, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were 5.19 ± 1.69 ng/mL, 3.10 ± 0.41 mmol/L, 6.26 ± 1.26 g/dL, 0.50 ± 0.07 mg/dL, 1.74 ± 0.09 mmol/L, 6.62 ± 0.81 nmol/ul, 926.20 ± 100.18 ug/dL, 51.17 ± 7.74 mIU/ml, and 143.16 ± 18.67 mIU/ml, respectively. The antioxidants activities of catalase, glutathione peroxidase (GPx) and superoxide dismutase (SOD) in the epididymal fluid were 121.55 ± 6.57 nmol/min/ml, 59.35 ± 10.98 nmol/min/ml and 0.18 ± 0.03 U/ml, respectively. Epididymal sperm motility and concentration were higher (P < 0.05) in the body and tail than the head. The viability indices of total and forward sperm motility, at 5 and 30 °C, obtained from the tail region were superior (P < 0.05) in both SHOTOR® and INRA-96® extenders than Green buffer extender. It may be concluded that INRA-96® extender is the best for storing dromedary epididymal spermatozoa at 5 and 30 °C.
从当地屠宰场在配种季节中分离出 35 只明显健康的单峰驼（Camelus dromedarius）的睾丸，年龄在 5 至 18 岁之间。使用 ELISA 商业试剂盒从一个附睾中收集附睾液，以测定 12 种生化和抗氧化参数。从另一个附睾的各个区域（头部、身体和尾部）收集精子，并在 5 和 30°C 下分别储存在 SHOTOR®、Green buffer®+20%卵黄和 INRA-96® 稀释液中。结果表明，在附睾液中，睾酮、葡萄糖、白蛋白、总蛋白、胆固醇、脂肪酸、铁、天门冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）的浓度分别为 5.19±1.69ng/mL、3.10±0.41mmol/L、6.26±1.26g/dL、0.50±0.07mg/dL、1.74±0.09mmol/L、6.62±0.81nmol/ul、926.20±100.18μg/dL、51.17±7.74mIU/ml 和 143.16±18.67mIU/ml。附睾液中过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的抗氧化活性分别为 121.55±6.57nmol/min/ml、59.35±10.98nmol/min/ml 和 0.18±0.03U/ml。与头部相比，身体和尾部的附睾精子活力和浓度更高（P<0.05）。在 SHOTOR®和 INRA-96®稀释液中，尾部获得的总精子活力和向前精子活力的存活率指数在 5 和 30°C 时均优于 Green buffer 稀释液（P<0.05）。因此可以得出结论，INRA-96®稀释液是在 5 和 30°C 下储存单峰驼附睾精子的最佳选择。
