Bustamante-Filho Ivan Cunha, Rosa Andrea P, Van der Linden Liana S, Pederzolli Carolina D, Neves Adriana P, Dutra-Filho Carlos S, Jobim Maria Inês M, Mattos Rodrigo C
Laboratório de Biotecnologia, UNIVATES, Rua Avelino Tallini, 171, Lajeado 95000-000, RS, Brazil.
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600, Anexo, Porto Alegre 90035-003, Brazil.
Anim Reprod Sci. 2014 Dec 30;151(3-4):164-8. doi: 10.1016/j.anireprosci.2014.10.015. Epub 2014 Nov 4.
The use of stallion semen collected from cauda epididymis for AI has increased due to the new protocols available for cryopreservation. Preserving the genetic material from valuable males that suffer sudden death or other events that prematurely end the stallion's reproductive life is an important strategy for Stud breeding management. While protecting spermatozoa from oxidative stress and infectious agents, the epididymis promotes the enhancement of sperm cell morphology and changes in membrane protein profile, increasing its fertility potential. The epididymal fluid must be a balanced redox environment to allow sperm preservation and protein-protein and protein-lipids interactions to quantify. The aim of this study was quantify the enzymatic ROS scavengers in epididymal fluid of pony and miniature breed stallions. Epididymides from 8 pony stallions and 12 miniature breed stallions were dissected and fluid from caput, corpus and cauda epididymis collected. Spermatozoa were separated of epididymal fluid by 2-step centrifugation. The activities of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured and compared between stallion groups and epididymal regions. The three enzymes were present in all epididymal regions tested, with higher activities of catalase and SOD in cauda epididymis in miniature breed stallions (P<0.05). GPx activity was higher in caput epididymis in pony stallions (P<0.05), however with no difference to fluid from cauda epididymis of both breeds. These results show a difference in antioxidant enzymatic scavengers between pony and miniature breed stallions. Also, our data confirm the protective role of cauda epididymis, preserving spermatozoa integrity from oxidative damage. As glutathione peroxidase is involved in several signaling pathways, its constant activity during epididymal transit corroborates the importance of this enzyme for spermatozoa maturation.
由于有了新的冷冻保存方案，从附睾尾部采集的种马精液用于人工授精的情况有所增加。保存因突然死亡或其他过早结束种马繁殖生命的事件而死亡的珍贵雄性种马的遗传物质，是种马场繁殖管理的一项重要策略。附睾在保护精子免受氧化应激和感染因子影响的同时，还能促进精子细胞形态的改善和膜蛋白谱的变化，提高其生育潜力。附睾液必须是一个平衡的氧化还原环境，以确保精子的保存以及蛋白质 - 蛋白质和蛋白质 - 脂质相互作用的量化。本研究的目的是量化矮种马和小型品种种马附睾液中的酶促活性氧清除剂。解剖了8匹矮种马和12匹小型品种种马的附睾，并收集了附睾头、附睾体和附睾尾的液体。通过两步离心从附睾液中分离出精子。测量并比较了种马组和附睾区域之间过氧化氢酶、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。在所测试的所有附睾区域中均存在这三种酶，小型品种种马附睾尾中的过氧化氢酶和SOD活性较高（P<0.05）。矮种马附睾头中的GPx活性较高（P<0.05），但与两个品种附睾尾的液体无差异。这些结果表明矮种马和小型品种种马之间抗氧化酶清除剂存在差异。此外，我们的数据证实了附睾尾的保护作用，可保护精子完整性免受氧化损伤。由于谷胱甘肽过氧化物酶参与多种信号通路，其在附睾转运过程中的恒定活性证实了该酶对精子成熟的重要性。
