Department of Gastroenterology, First Affiliated Hospital, Anhui Medical University, The Key Laboratory of Digestive of Disease of Anhui Province, Hefei, China
Clin Chim Acta. 2012 Jan 18;413(1-2):30-3. doi: 10.1016/j.cca.2011.06.036. Epub 2011 Jul 8.
Oxidative stress could be a major contributing factor to the tissue injury that characterize inflammatory bowel disease (IBD). Homocysteine (HCY) could cause oxidative damage to the colon tissue in ulcerative colitis (UC) patients, melatonin (MLT) supplementation could reduce oxidative damage that caused by HCY in vitro and in vivo. In this study, we aimed to determine the levels of plasma HCY and MLT simultaneously in UC patients.
Collected the clinical data of 112 UC patients and 110 healthy controls (HC). The levels of plasma HCY and MLT were detected by HPLC-FD method. The levels of plasma folate, vitamin B(12) (VitB(12)) were detected by ELISA method.
The levels of plasma HCY in UC patients were significantly higher than that in HC (11.27±7.26 μmol/L vs. 8.19±4.81 μmol/L, P=0.000). The levels of plasma MLT in UC patients were significantly lower than that in HC (49.06±31.40 pg/ml vs. 64.28±41.16 pg/ml, P=0.008). The levels of plasma folate and VitB(12) in UC patients were lower than that in HC (7.64±1.95 nmol/L vs. 9.14±1.23 nmol/L, 108.64±32.22 pmol/L vs. 112.64±33.33 pmol/L, P<0.05). The levels of plasma HCY and MLT in UC patients were not correlated with either the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) or the disease activity, localization and duration of UC (P>0.05). The change of increasing levels of plasma MLT but decreasing levels of plasma HCY was shown in UC patients, however, the association between the levels of plasma MLT and HCY were not statistically significant (P>0.05).
The levels of plasma HCY were increased whereas the levels of plasma MLT were decreased in UC patients.
氧化应激可能是炎症性肠病（IBD）特征性组织损伤的主要致病因素。同型半胱氨酸（HCY）可导致溃疡性结肠炎（UC）患者结肠组织氧化损伤，褪黑素（MLT）补充可减少 HCY 引起的体内外氧化损伤。在这项研究中，我们旨在同时确定 UC 患者的血浆 HCY 和 MLT 水平。
收集 112 例 UC 患者和 110 例健康对照（HC）的临床资料。采用 HPLC-FD 法检测血浆 HCY 和 MLT 水平，ELISA 法检测血浆叶酸、维生素 B12（VitB12）水平。
UC 患者血浆 HCY 水平明显高于 HC（11.27±7.26 μmol/L 比 8.19±4.81 μmol/L，P=0.000）。UC 患者血浆 MLT 水平明显低于 HC（49.06±31.40 pg/ml 比 64.28±41.16 pg/ml，P=0.008）。UC 患者血浆叶酸和 VitB12 水平低于 HC（7.64±1.95 nmol/L 比 9.14±1.23 nmol/L，108.64±32.22 pmol/L 比 112.64±33.33 pmol/L，P<0.05）。UC 患者血浆 HCY 和 MLT 水平与红细胞沉降率（ESR）、C 反应蛋白（CRP）或 UC 的疾病活动度、定位和持续时间均无相关性（P>0.05）。UC 患者表现为血浆 MLT 水平升高而 HCY 水平降低，但两者之间的关联无统计学意义（P>0.05）。
UC 患者血浆 HCY 水平升高，MLT 水平降低。
