Kim Tae Kyun, Park Young Sook, Baik Haing-Woon, Jun Jin Hyun, Kim Eun Kyung, Sull Jae Woong, Sung Ho Joong, Choi Jin Woo, Chung Sook Hee, Gye Myung Chan, Lim Ju Yeon, Kim Jun Bong, Kim Seong Hwan
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
World J Gastroenterol. 2016 Sep 7;22(33):7559-68. doi: 10.3748/wjg.v22.i33.7559.
To determine adiponectin expression in colonic tissue of murine colitis and systemic cytokine expression after melatonin treatments and sleep deprivation.
The following five groups of C57BL/6 mice were used in this study: (1) group I, control; (2) group II, 2% DSS induced colitis for 7 d; (3) group III, 2% DSS induced colitis and melatonin treatment; (4) group IV, 2% DSS induced colitis with sleep deprivation (SD) using specially designed and modified multiple platform water baths; and (5) group V, 2% DSS induced colitis with SD and melatonin treatment. Melatonin (10 mg/kg) or saline was intraperitoneally injected daily to mice for 4 d. The body weight was monitored daily. The degree of colitis was evaluated histologically after sacrificing the mice. Immunohistochemical staining and Western blot analysis was performed using anti-adiponectin antibody. After sampling by intracardiac punctures, levels of serum cytokines were measured by ELISA.
Sleep deprivation in water bath exacerbated DSS induced colitis and worsened weight loss. Melatonin injection not only alleviated the severity of mucosal injury, but also helped survival during stressful condition. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. The expression levels of IL-6 and IL-17 were increased in the serum of mice with DSS colitis but decreased after melatonin injection.
This study suggested that melatonin modulated adiponectin expression in colonic tissue and melatonin and adiponectin synergistically potentiated anti-inflammatory effects on colitis with sleep deprivation.
确定褪黑素治疗和睡眠剥夺后小鼠结肠炎结肠组织中脂联素的表达及全身细胞因子的表达。
本研究使用了以下五组C57BL/6小鼠：（1）第一组，对照组；（2）第二组，用2%葡聚糖硫酸钠（DSS）诱导结肠炎7天；（3）第三组，用2% DSS诱导结肠炎并进行褪黑素治疗；（4）第四组，用2% DSS诱导结肠炎并使用专门设计和改良的多平台水浴进行睡眠剥夺（SD）；（5）第五组，用2% DSS诱导结肠炎并进行SD和褪黑素治疗。每天给小鼠腹腔注射褪黑素（10 mg/kg）或生理盐水，持续4天。每天监测体重。处死小鼠后，通过组织学评估结肠炎的程度。使用抗脂联素抗体进行免疫组织化学染色和蛋白质印迹分析。通过心脏穿刺取样后，用酶联免疫吸附测定法（ELISA）测量血清细胞因子水平。
水浴中的睡眠剥夺加剧了DSS诱导的结肠炎并加重了体重减轻。注射褪黑素不仅减轻了黏膜损伤的严重程度，还有助于在应激状态下存活。脂联素在结肠炎黏膜中的表达水平降低，在合并睡眠剥夺的结肠炎中观察到最低水平。注射褪黑素显著（P < 0.05）恢复了脂联素的表达。DSS结肠炎小鼠血清中白细胞介素-6（IL-6）和白细胞介素-17（IL-17）的表达水平升高，但注射褪黑素后降低。
本研究表明，褪黑素调节结肠组织中脂联素的表达，褪黑素和脂联素协同增强对合并睡眠剥夺的结肠炎的抗炎作用。
