Translational Research Unit, Hospital General de Ciudad Real, Spain.
Behav Brain Res. 2011 Nov 20;225(1):71-6. doi: 10.1016/j.bbr.2011.06.034. Epub 2011 Jul 7.
Heat-shock proteins play functional roles on brain regulatory processes which are deeply involved in drug addiction, such as synaptic plasticity. However, few studies have been focused on gene expression of heat-shock proteins (Hsp) as potential diagnostic tools for addiction risk. This work tries to provide new knowledge on this field by using two rat models of differential vulnerability to morphine addiction in order to study differential gene expression of a selected group of Hsp genes in the nucleus accumbens (NAc). Hsp70-1A, 84, 86 and 105 genes were similarly regulated by an acute injection of morphine in two subpopulations of Sprague Dawley (SD) rats showing different rates of extinction of morphine conditioned preference. However, Lewis and Fischer rats, two strains that differ in many aspects of drug seeking behaviours, exhibited marked differences in their expression patterns of Hsp84, 86 and 105. These results suggest that differential Hsp gene expression could be related to addiction vulnerability and recommend further work to validate these proteins as potential markers for drug addiction risk.
热休克蛋白在大脑调节过程中发挥功能作用,这些过程与药物成瘾密切相关,如突触可塑性。然而,很少有研究关注热休克蛋白(Hsp)的基因表达作为成瘾风险的潜在诊断工具。本研究使用两种对吗啡成瘾易感性不同的大鼠模型,试图在该领域提供新知识,以研究选定的一组 Hsp 基因在伏隔核(NAc)中的差异基因表达。在两组表现出不同的吗啡条件性偏好消退率的 Sprague Dawley(SD)大鼠亚群中,Hsp70-1A、84、86 和 105 基因均被单次吗啡注射类似调节。然而,Lewis 和 Fischer 大鼠是在许多药物寻求行为方面存在差异的两个品系,其 Hsp84、86 和 105 的表达模式存在明显差异。这些结果表明,差异的 Hsp 基因表达可能与成瘾易感性有关,并建议进一步验证这些蛋白质作为药物成瘾风险的潜在标志物。