Wilschut Karlijn J, van Tol Helena T A, Arkesteijn Ger J A, Haagsman Henk P, Roelen Bernard A J
Department of Farm Animal Health, Faculty of Veterinary Medicine, Yalelaan 104, 3584 CM, Utrecht University, Utrecht, The Netherlands.
Stem Cell Res. 2011 Sep;7(2):112-23. doi: 10.1016/j.scr.2011.05.001. Epub 2011 May 10.
A muscle progenitor cell population, other than muscle satellite cells, can be isolated and purified from porcine muscle tissue. We show the presence of at least two types of stem cells in porcine muscle: those that express α6 integrin and those that lack expression of this integrin type. By flow cytometry, we could select for myogenic stem cell populations expressing the neural cell adhesion molecule in the presence and absence of α6 integrin. The expression of α6 integrin showed an advantage in the formation of myotubes, possibly by an improved cell fusion capacity. This notion was strengthened by qRT-PCR analysis showing sustained PAX7, MYF5 and DESMIN expression and a strong myogenic differentiation capacity of this stem cell population. Selective inhibition of α6 integrin function, both by blocking antibodies and RNA interference, showed the importance of α6 integrin in myogenic differentiation of muscle stem cells. It is concluded that α6 integrin expression can be used as biomarker to select for highly myogenic cell populations in muscle tissue.
除了肌肉卫星细胞外，还可以从猪肌肉组织中分离并纯化出一种肌肉祖细胞群体。我们发现猪肌肉中至少存在两种类型的干细胞：表达α6整合素的干细胞和不表达这种整合素类型的干细胞。通过流式细胞术，我们能够在α6整合素存在和不存在的情况下，筛选出表达神经细胞黏附分子的成肌干细胞群体。α6整合素的表达在肌管形成方面具有优势，这可能是通过提高细胞融合能力实现的。qRT-PCR分析显示该干细胞群体中PAX7、MYF5和结蛋白持续表达且具有很强的成肌分化能力，这一观点得到了进一步证实。通过阻断抗体和RNA干扰对α6整合素功能进行选择性抑制，结果表明α6整合素在肌肉干细胞的成肌分化中具有重要作用。得出的结论是，α6整合素的表达可以用作生物标志物，以筛选肌肉组织中具有高度成肌能力的细胞群体。
