Ding Shijie, Wang Fei, Liu Yan, Li Sheng, Zhou Guanghong, Hu Ping
Key Laboratory of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Cell Biology, Center of Excellence in Molecular and Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Cell Death Discov. 2017 Apr 10;3:17003. doi: 10.1038/cddiscovery.2017.3. eCollection 2017.
Pig is an important food source and an excellent system to model human diseases. Careful characterization of the swine skeletal muscle stem cells (satellite cells) will shed lights on generation of swine skeletal muscle disease model and efficient production of porcine meat for the food industry. Paired box protein 7 (Pax7) is a highly conserved transcription factor shared by satellite cells from various species. However, the sequence of Pax7 has not been characterized in pig. The lack of method to isolate highly purified satellite cells hinders the thorough characterization of the swine satellite cells. Here we found molecular markers for swine satellite cells and revealed that the porcine satellite cells were heterogeneous in various pieces of skeletal muscle. We further developed a method to isolate highly purified satellite cells directly from porcine muscles using fluorescence-activated cell sorting. We next characterized the proliferation and differentiation abilities of isolated satellite cells ; and found that long-term culturing of satellite cells led to stemness loss.
猪是一种重要的食物来源，也是模拟人类疾病的优良模型系统。对猪骨骼肌干细胞（卫星细胞）进行细致的特征描述，将有助于建立猪骨骼肌疾病模型，并为食品工业高效生产猪肉。配对盒蛋白7（Pax7）是一种在不同物种的卫星细胞中高度保守的转录因子。然而，猪的Pax7序列尚未得到鉴定。缺乏分离高度纯化卫星细胞的方法阻碍了对猪卫星细胞的全面特征描述。在此，我们发现了猪卫星细胞的分子标志物，并揭示猪卫星细胞在不同骨骼肌中具有异质性。我们进一步开发了一种利用荧光激活细胞分选技术直接从猪肌肉中分离高度纯化卫星细胞的方法。接下来，我们对分离出的卫星细胞的增殖和分化能力进行了特征描述，发现卫星细胞的长期培养会导致干性丧失。
