用于人胚胎干细胞/人诱导多能干细胞衍生和培养的人饲养层细胞系。
Human feeder cell line for derivation and culture of hESc/hiPSc.
作者信息
McKay Tristan R, Camarasa Maria V, Iskender Banu, Ye Jinpei, Bates Nicola, Miller Duncan, Fitzsimmons Jayne C, Foxler Daniel, Mee Maureen, Sharp Tyson V, Aplin John, Brison Daniel R, Kimber Susan J
机构信息
North West Embryonic Stem Cell Centre, Faculty of Life Sciences, Core Technology Facility, University of Manchester, 46 Grafton Street, Manchester, M13 9NT, UK.
出版信息
Stem Cell Res. 2011 Sep;7(2):154-62. doi: 10.1016/j.scr.2011.04.005. Epub 2011 May 16.
We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.
我们从妊娠中期早期的胎盘基质成纤维细胞（ihPSF）中生成了一种稳定过表达多梳蛋白BMI-1的人饲养细胞系。这些饲养细胞具有在长期培养中维持人胚胎干细胞（hESc）的能力，而hTERT或BMI-1/hTERT永生化饲养细胞系则不具备这种能力。ihPSF能够在近乎无动物成分（不含非人类动物成分）的条件下支持新的hESc系的衍生，并在临床使用所需的完全无动物成分条件下支持新衍生的hESc和人诱导多能干细胞（hiPS）系的持续培养。
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