Zhao Zhuran, Chen Xi, Wang Shan, Fu Min, Shen Huan, Li Jiayu, Xu Jun, Qin Jiong, Shi Cheng
Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Department of Pediatrics, Peking University People's Hospital, Beijing, 100044, China.
Cell Biosci. 2025 May 30;15(1):72. doi: 10.1186/s13578-025-01410-0.
Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, we report an optimized, highly efficient protocol by utilizing chemically defined and xeno-free media for the derivation of human extended pluripotent stem cell lines from discarded blastocysts. With this method, we successfully isolated hEPS cell lines from discarded blastocysts with an efficiency of 46%. Chemically defined and xeno-free media simplified the process of hEPS cell isolation with a higher survival rate of cell aggregation passaging from outgrowth. To our knowledge, this is the first report of hEPS cells being efficiently derived from discarded blastocysts under chemically defined and xeno-free conditions.
The online version contains supplementary material available at 10.1186/s13578-025-01410-0.
据报道，人类扩展多能干细胞（hEPS）源自废弃囊胚，然而在此方案中，在饲养层和动物血清条件下hEPS细胞的诱导方法极其复杂，这也限制了hEPS的安全性和均一性。在此，我们报告一种优化的高效方案，利用化学成分明确且无动物成分的培养基，从废弃囊胚中诱导生成人类扩展多能干细胞系。通过这种方法，我们成功地从废弃囊胚中分离出hEPS细胞系，效率为46%。化学成分明确且无动物成分的培养基简化了hEPS细胞的分离过程，细胞集落传代时细胞聚集的存活率更高。据我们所知，这是首次报道在化学成分明确且无动物成分的条件下，能从废弃囊胚中高效诱导生成hEPS细胞。
在线版本包含可在10.1186/s13578-025-01410-0获取的补充材料。
