State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China.
Int J Biol Macromol. 2011 Nov 1;49(4):688-92. doi: 10.1016/j.ijbiomac.2011.06.030. Epub 2011 Jul 7.
The one-pot synthesis and characterization of cross-linked quaternized chitosan microspheres (CQCM) as a protein adsorbent are presented. First of all, chitosan particles were prepared by spray drying method, and then they were quaternized and cross-linked in turn with glycidyltrimethylammonium (GTMAC) chloride and glutaraldehyde in isopropanol containing 10% water in one-pot. The effect of the reaction temperature, reaction time and the amounts of added GTMAC and glutaraldehyde on the protein adsorption ability of CQCM was investigated. The adsorption behavior of the CQCM prepared in the optimum synthetic conditions was well described by the Langmuir isotherm with maximum adsorption capacity equal to 1424 mg BSA/g dry weight. The particle size ranged from 7.6 to 48.9 μm. The mechanism of adsorption-desorption of BSA to the CQCM was ion-exchange. Finally, the extraction of soybean peroxidase from crude soybean peroxidase solution using the CQCM was performed.
本文介绍了一种通过一锅法合成交联季铵化壳聚糖微球（CQCM）作为蛋白质吸附剂的方法。首先，通过喷雾干燥法制备壳聚糖颗粒，然后在异丙醇中含有 10%水的条件下，用氯化三甲铵（GTMAC）和戊二醛依次对其进行季铵化和交联。考察了反应温度、反应时间以及添加的 GTMAC 和戊二醛的量对 CQCM 蛋白质吸附能力的影响。在最佳合成条件下制备的 CQCM 的吸附行为很好地符合朗缪尔等温线，最大吸附容量等于 1424mg BSA/g 干重。颗粒尺寸范围为 7.6 至 48.9μm。BSA 与 CQCM 的吸附-解吸机制为离子交换。最后，使用 CQCM 从粗大豆过氧化物酶溶液中提取大豆过氧化物酶。
