Department of Applied Chemistry, College of Science, Northwestern Polytechnical University, Xi'an 710072, China.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4401-8. doi: 10.1016/j.msec.2013.06.038. Epub 2013 Jul 1.
BSA/Fe3O4 magnetic composite microspheres with high saturation magnetization and paramagnetic property were prepared via inverse emulsion technology at room temperature, bovine serum albumin (BSA, 60 KD), magnetic nanoparticles (Fe3O4) and glutaraldehyde as macromonomer, inorganic particles and cross-linking agent, respectively. Fourier transform infrared (FTIR), scanning electron microscope (SEM), metalloscope, and particle size analyzer were used to characterize morphology and structure of composite microspheres. Vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA) were used to test magnetic properties of the synthesized samples, adsorption capacity of microspheres was determined by ultraviolet spectrophotometer (UV). The results showed that BSA/Fe3O4 microspheres were 43 μm with relatively narrow particle size distribution, perfect sphere-shaped morphologies, superparamagnetism with a saturation magnetization of 11 emu/g, and high magnetic content with a value of 57.29%. The main factors influencing properties of microspheres including raw material ratio, the amount of emulsifier and cross-linking agent, agitation speed were investigated and optimized. Furthermore, these microspheres accompanying with high separable and reusable efficient may have great potential application in the field of separation, in particular, removal of antibiotics. Adsorption capacities of the microspheres of four different kinds of antibiotics (erythromycin, streptomycin, tetracycline and chloramphenicol) ranging from 69.35 mg/g to 147.83 mg/g were obtained, and Langmuir isotherm model coincided with equilibrium data than that of the Freundlich model.
BSA/Fe3O4 磁性复合微球具有高饱和磁化强度和顺磁性，通过室温下的反相乳液技术制备，牛血清白蛋白（BSA，60KD）、磁性纳米粒子（Fe3O4）和戊二醛分别作为大分子单体、无机颗粒和交联剂。傅里叶变换红外（FTIR）、扫描电子显微镜（SEM）、金相显微镜和粒度分析仪用于表征复合微球的形貌和结构。振动样品磁强计（VSM）和热重分析（TGA）用于测试合成样品的磁性，微球的吸附能力通过紫外分光光度计（UV）测定。结果表明，BSA/Fe3O4 微球粒径分布较窄，为 43μm，呈较规则的球形，具有超顺磁性，饱和磁化强度为 11emu/g，磁含量高，为 57.29%。考察并优化了影响微球性能的主要因素，包括原料比、乳化剂和交联剂用量、搅拌速度等。此外，这些微球具有高分离和可重复使用的效率，可能在分离领域，特别是抗生素去除方面具有巨大的潜在应用价值。微球对四种不同抗生素（红霉素、链霉素、四环素和氯霉素）的吸附能力在 69.35mg/g 到 147.83mg/g 之间，Langmuir 等温线模型比 Freundlich 模型更符合平衡数据。
