仿生方法修复牙本质基质蛋白 1 和牙髓干细胞穿孔。
Biomimetic approach to perforation repair using dental pulp stem cells and dentin matrix protein 1.
机构信息
Department of Endodontics, University of Illinois at Chicago, Chicago, Illinois, USA.
出版信息
J Endod. 2011 Aug;37(8):1092-7. doi: 10.1016/j.joen.2011.05.019.
Abstract
INTRODUCTION
Dentin regeneration could be an ideal treatment option to restore tissue function. This study was conducted to evaluate the ability of dental pulp stem cells (DPSCs) and dentin matrix protein 1 (DMP1) impregnated within a collagen scaffold to regenerate dentin.
METHODS
Simulated perforations were created in 18 dentin wafers made from freshly extracted human molars. Six groups were established. They were (1) empty wafers, (2) mineral trioxide aggregate, (3) collagen scaffold, (4) scaffold with DMP1, (5) scaffold with DPSCs, and (6) scaffold with DPSCs and DMP1. One sample was placed subcutaneously in each mouse with three mice in each group. After 12 weeks, the samples were subjected to radiographic, histological, and immunohistochemical evaluations.
RESULTS
DPSCs impregnated within a collagen scaffold differentiated into odontoblast-like cells forming a highly cellular, vascular, and mineralized matrix in the presence of DMP1.
CONCLUSIONS
A triad consisting of DPSCs, DMP1, and a collagen scaffold promotes dentin regeneration in a simulated perforation repair model.
摘要
简介
牙本质再生可能是恢复组织功能的理想治疗选择。本研究旨在评估牙髓干细胞（DPSCs）和浸渍在胶原支架内的牙本质基质蛋白 1（DMP1）再生牙本质的能力。
方法
在 18 个由新鲜提取的人磨牙制成的牙本质片上模拟穿孔。建立了 6 组。它们分别为：（1）空片，（2）三氧化物聚合体，（3）胶原支架，（4）DMP1 浸渍的支架，（5）DPSCs 浸渍的支架，以及（6）DPSCs 和 DMP1 浸渍的支架。每组 3 只小鼠，每只小鼠皮下植入 1 个样本。12 周后，对样本进行放射学、组织学和免疫组织化学评估。
结果
在 DMP1 的存在下，浸渍在胶原支架内的 DPSCs 分化为成牙本质样细胞，形成了一个高度细胞化、血管化和矿化的基质。
结论
由 DPSCs、DMP1 和胶原支架组成的三联体在模拟穿孔修复模型中促进牙本质再生。
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